Syringin production and related secondary metabolism enzyme activities in suspension cultures of Saussurea medusa treated with different elicitors (yeast extract, chitosan and Ag + ) were investigated. All elicitors enhanced syringin production, and the optimal feeding protocol was the combined addition of 1.5%
The culture of Saussurea medusa cell were cultured in an internal loop airlift bioreactor with sifter draft tube (ILABSDT) was investigated. Under the optimal culture conditions, which were inoculation size 1.5 g(d.m.) dm -3 , aeration rate 0.3 dm 3 (air) dm -3 (medium) min -1 , and 14 mesh sifter holes, the maximum biomass, syringin content and syringin production reached 11.7 g(d.m.) dm -3 , 17.7 mg g -1 and 206.6 mg dm -3 , respectively. Among cell cultures in shake flask, bubble column bioreactor and ILABSDT, ILABSDT had the highest syringin productivity and reached 12.41 mg dm -3 d -1 .Additional key words: aeration rate, inoculation size, mesh, sifter draft tube.
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