Elicitor-enhanced syringin production in suspension cultures of Saussurea medusa

Abstract

Syringin production and related secondary metabolism enzyme activities in suspension cultures of Saussurea medusa treated with different elicitors (yeast extract, chitosan and Ag + ) were investigated. All elicitors enhanced syringin production, and the optimal feeding protocol was the combined addition of 1.5%

Enhanced production of silymarin by Ag+elicitor in cell suspension cultures ofSilybum marianum

“…A similar observation was obtained in suspension cultures of Saussurea medusa Maxim and Linum album Boiss (Xu et al, 2007;Shams-Ardakani et al, 2005). In view of the positive effects of manipulation of the components of culture medium, the growth and flavonolignan production of S. marianum cell suspension were tested using different concentrations of KNO 3 , KH 2 PO 4 , iron, and calcium.…”

“…The mechanisms of elicitation of secondary metabolite production by cell suspension cultures are not fully understood. However, many studies suggest that an elicitor signal is perceived by a receptor on the surface of the plasma membrane and initiates a signal transduction network, which regulates the expression of biosynthesis genes involved in plant secondary metabolism and further key enzymes that catalyze the biosynthesis of the target secondary metabolites (Xu et al, 2007;Zhao et al, 2005).…”

“…In plant cell cultures, the use of elicitors has been an important strategy for improving the production of plant secondary metabolites. Elicitors can trigger an array of defense or stress responses and activate specific genes for the enzymes involved in secondary metabolite biosynthesis, and improve the production of plant secondary metabolites (Namdeo, 2007;Rajendran et al, 1994;Xu et al, 2007). In general, elicitors are classified on the basis of their origin and molecular structure.…”

The effects of elicitation on the expression of key enzyme genes and on production of tropane alkaloids in Anisodus acutangulus plant

“…It is also possible that the elicitation effects might be due to other components, still not being identified (Ertola & Hours 1998). In many species it has been proven to stimulate or decrease different secondary metabolites, such as syringin, valeportriates, podophyllotoxin and silymarin (Kittipongpatana et al 2002;Shams-Ardakani et al 2005;Xu et al 2007;Hasanloo et al 2009). Our current work revealed that YE is a stimulating factor for TA production in A. acutangulus plant, which gradually boosted the contents of hyoscyamine and scopolamine in the root and stem in the range of 96 h treatment, the same as the key genes of TA biosynthesis.…”

“…Lots of experiments have demonstrated that MJ was able to stimulate the production of several compounds in many species (Ignatov et al 1996;Rijhwani & Shaks 1998;Dong & Zhong 2002;Wang & Zhong 2002;Vasconsuelo & Boland 2007). Yeast extract (YE), a kind of polysaccharide, was also been reported to have the ability to regulate increase or decrease different secondary metabolites in many species, such as syringin, valeportriates, podophyllotoxin and silymarin (Kittipongpatana et al 2002;Shams-Ardakani et al 2005;Xu et al 2007;Hasanloo et al 2009). Abscisic acid (ABA), also called resistant inducer element, is a key element which can balance the plant endogenous hormones and is relevant to growth-active substances.…”