Poor HRQOL was associated with a number of factors among IDUs at methadone clinics in Taipei, Taiwan. To improve HRQOL in this population, future programs should focus on IDUs with a history of drug overdose. In addition, methadone programs and social support should be integrated to improve HRQOL among this socially marginalized population.

Background: Inflammatory responses from chronic infection might affect the brain and increase the risk of depressive disorder. However, the temporal association between chronic infection (eg, tuberculosis [TB]) and incident depressive disorder has not been prospectively evaluated.

Abstract. This study presents a new epistemological analysis of drought chronology through a well-defined methodology for reconstructing past drought series, as well as series of other associated ecological and societal impact variables. Instead of building a grading system based on mixed criteria, this method facilitates transparency in the reconstruction process and enables the statistical examination of all variables when building series. The data for the present study are derived mainly from the REACHES (Reconstructed East Asian Climate Historical Encoded Series) database; however, other archival documentary and index data from independent sources are also applied to understand drought narratives and to cross-check and validate the analysis derived from REACHES. From the time series analysis, six severe drought periods are identified in the Qing dynasty, and then a spatial analysis is performed to demonstrate the spatial distribution of drought and other variables in the six periods, as well as a social network analysis to reveal connections between drought and other ecological and societal variables. Research results clearly illustrate the role of human intervention in influencing the impacts of drought and their societal consequences. Particularly, the correlation between drought and socioeconomic turmoil is not strong; crop failure and famine are important intermediate factors, while ecological factors such as locust and disaster relief measures are all imperative to intervene between crop production and famine. Implications of the study on drought impact are provided, as well as the significance of drought on historical climate reconstruction studies.

TSG101 (tumor susceptibility gene 101) is a multi-domain protein known to act in the cell nucleus, cytoplasm, and periplasmic membrane. Remarkably, TSG101, whose location within cells varies with the stage of the cell cycle, affects biological events as diverse as cell growth and proliferation, gene expression, cytokinesis, and endosomal trafficking. The functions of TSG101 additionally are recruited for viral and microvesicle budding and for intracellular survival of invading bacteria. Here we report that the TSG101 protein also interacts with and down-regulates the promoter of the p21CIP1/WAF1tumor suppressor gene, and identify a p21 locus and TSG101 domains that mediate this interaction. TSG101 deficiency in Saos-2 human osteosarcoma cells was accompanied by an increased abundance of p21 mRNA and protein and the retardation of cell proliferation. A cis-acting element in the p21 promoter that interacts with TSG101 and is required for promoter repression was located using chromatin immunoprecipitation (ChIP) analysis and p21-driven luciferase reporter gene expression, respectively. Additional analysis of TSG101 deletion mutants lacking specific domains established the role of the central TSG101 domains in binding to the p21 promoter and demonstrated the additional essentiality of the TSG101 C-terminal steadiness box (SB) in the repression of p21 promoter activity. Neither binding of TSG101 to the p21 promoter nor repression of this promoter required the TSG101 N-terminal UEV domain, which mediates the ubiquitin-recognition functions of TSG101 and its actions as a member of ESCRT endocytic trafficking complexes, indicating that regulation of the p21 promoter by TSG101 is independent of its role in such trafficking.

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