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To simulate in vitro the conditions under which enzymes act in vivo, enzyme molecules have been entrapped in hydrated reverse micelles of a surfactant in organic solvents. In this system the catalytic activity of one of the enzymes studied (peroxidase) became much higher than in water, and the specificity of the other enzyme (alcohol dehydrogenase) was dramatically altered.

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Regioselective Enzymatic Acylation as a Tool for Producing Solution-Phase Combinatorial Libraries. -The flavonoid bergenin (I) is used as suitable model substrate for the title investigations. 11 Enzymes are identified as possible catalysts for selective acylation at the different hydroxyl sites. The strategy leads to a library of 167 selectively acylated derivatives (produced on a robotic workstation in a 96-well plate format).-(MOZHAEV, V. V.; BUDDE, C. L.; RICH, J. O.; USYATINSKY, A. YA.; MICHELS, P. C.; KHMELNITSKY, YU. L.; CLARK, D. S.; DORDICK, J. S.; Tetrahedron 54 (1998) 16, 3971-3982; EnzyMed, Inc., Iowa City, IA 52242, USA; EN)