Studies of mesenteric lymph nodes of rats by indirect immunoperoxidase method using monoclonal antibodies to cytochrome P450 1A/1A2 after oral dose of benzo[a]pyrene showed the presence of these cytochrome forms in monocytes, macrophages, reticular and litoral cells, cell detritus, and liquid contents of the paracortical zone and medullary substance sinuses. Oxidation of various exo- and endogenous toxins in the lymph nodes was revealed.
The expression of molecular markers characterizing activity of the tumor process and metastases (proliferation marker Ki-67, angiogenesis marker CD34, and lymphangiogenesis markers podoplanin and LYVE-1) was assessed by immunohictochemical method in the primary tumor specimens collected during surgery for cutaneous melanoma (40 patients). Proliferative activity of the tumor tissue and volume density of peritumoral blood and lymph vessels increased with increasing tumor malignancy, which could indicate the risk of metastases.
In control rats and animals receiving polyphenol compounds extracted from Alchemilla vulgaris, intense cooling stimulated synthesis of thyroid hormones and promoted their peripheral deiodination. In control rats, the period of recovery after cooling (readaptation period) was characterized by a compensatory reduction in thyroid function. In rats receiving test preparation, this period was characterized by significant activation of thyroid hormone synthesis and by further growth of reserve follicles that appeared during the cooling.
Key Words: polyphenol compounds; thyroid gland," morphometry; thyroid hormones; coolingNegative anthropogenic factors of modern life reduce individual and population health capacities, increase the incidence of specific pathology, and contribute to the development of new diseases caused by ecological stress [ 1 ]. Treatment with plant preparations is one of the most efficient ways of increasing body resistance [4][5][6][7]9]. Active components of many phytopreparations are represented by polyphenol compounds (PPC), characterized by a wide spectrum of biological activities and low toxicity [2]. Neurohumoral mechanisms and, in particular the effects of thyroid hormones play an important role in increasing the body resistance to nonspecific stress factors [5].In this study we investigated the effects of PPC extracted from the overground part ofAlchemilla vulgaris on the functional activity of the thyroid gland in rats exposed to extreme cooling.
MATERIALS AND METHODSThe PPC preparation from the overground part of Alchemilla vulgaris was prepared in the
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