Fluorescent microscopic imaging with the help of small-molecule
probes (chemoprobes) is one of the most feasible approaches for noninvasive
sensing of intracellular molecules. However, the “always on”
property of current chemoprobes failed to achieve time-resolved monitoring.
Here, we report the development of a supramolecular nanoassembling
strategy to integrate multiple functions on one nanoscale probe (nanoprobe)
with a cyclical on–off switchable sensing ability. The reversal
of the nanoprobe can be rapidly achieved by converting the single-wavelength
near-infrared (NIR) laser to two-way emissions by a lanthanum nanoparticle
core that is sensitive to the light power density. Through regulating
the NIR power density, the azobenzene derivative, which was doped
in the surface of the lipid bilayer of the nanoprobe, can act as an
“impeller” and “brake” for bio-benign
activation and deactivation, respectively, of the nanoprobe in biological
applications. A reduced nicotinamide adenine dinucleotide nanoprobe
was constructed as the model to demonstrate precise and time-resolved
monitoring of intracellular processes including cancerous glycolysis
and ligand-induced enzymatic processes. We envision that this cyclical
on–off switchable nanoprobe strategy will hold great promise
for endowing universal chemoprobes with high precision and temporal
resolution.
Upon EBV lytic reactivation, the virus-encoded DNA replication machinery functions in the nucleus, while the newly synthesized DNA is encapsidated and transported to the cytoplasm for final virus assembly. The single-stranded DNA binding protein BALF2 executing functions within the nucleus was also identified in the tegument layer of mature virions.
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