Arsenite-oxidizing and arsenate-reducing bacteria associated with arsenic-rich groundwater in Taiwan

Liao, Vivian Hsiu‐Chuan; Chu, Yen-Ping; Su, Yu-Chen; Hsiao, Sheng‐Mou; Wei, Chia-Cheng; Liu, Chen‐Wuing; Liao, Chung‐Min; Shen, Wei-Chiang; Chang, Fi-John

doi:10.1016/j.jconhyd.2010.12.003

Cited by 185 publications

(101 citation statements)

References 49 publications

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“…P. stutzeri GIST-BDan2 (EF429003) is an As(III)-oxidizing strain isolated from constructed wetlands (Chang et al, 2010). Three arsenate reducers of Pseudomonas were isolated in As-rich groundwater (Liao et al, 2011). Pseudomonas sp.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies showed that the As(V)-reducing bacterium, Citrobacter sp. AR-7, almost completely reduced As(V) to As(III) with 3 hr incubation (833.33 μmol/(L·hr)) (Liao et al, 2011), Bacillus sp. SXB reduced 95% As(V) of 1 mmol/L in 22 hr (43.18 μmol/(L·hr)), and Pantoea sp.…”

Section: As(iii)-oxidizing Bacteriamentioning

confidence: 98%

“…The ability of the obtained As-resistant bacteria to oxidize As(III) was tested using a qualitative AgNO 3 screening method as described by Liao et al (2011). Briefly, exponential-phase culture was centrifuged and then resuspended in CDM containing 1 mmol/L As(III).…”

Section: Selection Of As(iii)-oxidizing Strainmentioning

confidence: 99%

“…This result may suggest a significant degree of novelty in the genes encoding enzymatic As(V) reduction in HN-2. Several studies observed that despite clear evidence of As-transforming activity by microorganisms, no amplicon for arsenite oxidase (aoxB), As(V) respiratory reductase (arrA) or arsenate reductase (arsC) was attained using the reported PCR primers and protocols (Handley et al, 2009;Liao et al, 2011). Liao et al isolated three arsenate-reducing strains of Pseudomonas, and PCR failed to amplify any As marker genes for the two Pseudomonas sp.…”

Section: As(iii)-oxidizing Bacteriamentioning

confidence: 99%

“…Results indicated that the isolated strain behaved differently at different levels of DO, while pH and ORP kept relatively steady. It was reported by Liao et al (2011) that an isolated As-resistant bacteria showed different As tolerance and that As(V)-reducing bacteria showed different As(V) reduction rates under aerobic and anoxic conditions. Thus, it is possible that bacteria behave distinctly at different DO because of diverse metabolic mechanisms in different growth conditions.…”

Section: As(iii)-oxidizing Bacteriamentioning

confidence: 99%

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Arsenic redox transformation by Pseudomonas sp. HN-2 isolated from arsenic-contaminated soil in Hunan, China

Zhang

Yin

Cai

et al. 2016

Journal of Environmental Sciences

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A mesophilic, Gram-negative, arsenite[As(III)]-oxidizing and arsenate[As(V)]-reducing bacterial strain, Pseudomonas sp. HN-2, was isolated from an As-contaminated soil. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the strain was closely related to Pseudomonas stutzeri. Under aerobic conditions, this strain oxidized 92.0% (61.4 μmol/L) of arsenite to arsenate within 3 hr of incubation. Reduction of As(V) to As(III) occurred in anoxic conditions. Pseudomonas sp. HN-2 is among the first soil bacteria shown to be capable of both aerobic As(III) oxidation and anoxic As(V) reduction. The strain, as an efficient As(III) oxidizer and As(V) reducer in Pseudomonas, has the potential to impact arsenic mobility in both anoxic and aerobic environments, and has potential application in As remediation processes.

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Section: Discussionmentioning

confidence: 99%

Section: As(iii)-oxidizing Bacteriamentioning

confidence: 98%

Section: Selection Of As(iii)-oxidizing Strainmentioning

confidence: 99%

Section: As(iii)-oxidizing Bacteriamentioning

confidence: 99%

Section: As(iii)-oxidizing Bacteriamentioning

confidence: 99%

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Arsenic redox transformation by Pseudomonas sp. HN-2 isolated from arsenic-contaminated soil in Hunan, China

Zhang

Yin

Cai

et al. 2016

Journal of Environmental Sciences

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Bioremediation of Water: A Sustainable Approach

Chakraborty

Sikder

Mukherjee

et al. 2013

Sustainable Development in Chemical Engineering Innovative Technologies

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Arsenic Removal and Biotransformation Potential of Exiguobacterium Isolated From an Arsenic‐Rich Soil of Chhattisgarh, India

Pandey

Bhatt

2015

CLEAN Soil Air Water

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The presence of arsenic (As) in soil and groundwater has become a serious environmental issue of various regions of India. A haloalkaliphilic Exiguobacterium sp. (As-9) was isolated via enrichment culture technique from an arsenic-rich soil of the Rajnandgaon district in the state of Chhattisgarh, India, and characterized as a novel arsenic removing bacteria. The isolate proved to be capable of growing over a broad range of arsenic concentration and was resistant to relatively high levels of arsenate (700 mM) and arsenite (180 mM). It displayed great potential in removing about 99% arsenic under aerobic conditions from the aqueous environment in ten days at 37°C at 100 rpm. Electron microscopy revealed a fourfold increase in the cell size due to intracellular accumulation of arsenic. Selective enzyme assays confirmed that the two intracellular enzymes, namely arsenate reductase and arsenite oxidase played a significant role in arsenate reduction and arsenite oxidation, respectively, thereby contributing to the effective removal of arsenic. These results provide evidence for the occurrence of two distinct mechanisms involved in arsenic resistance; one via arsenate reduction and the other via arsenite oxidation. Moreover, the study reports for the first time the role of arsenic transforming bacteria from this region which could resist and transform exceptionally high concentrations of arsenic oxyanions. Such metabolically active bacterial strain may find application in the treatment of arsenic contaminated soil and aquifers in the near future.

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Arsenite-oxidizing and arsenate-reducing bacteria associated with arsenic-rich groundwater in Taiwan

Cited by 185 publications

References 49 publications

Arsenic redox transformation by Pseudomonas sp. HN-2 isolated from arsenic-contaminated soil in Hunan, China

Arsenic redox transformation by Pseudomonas sp. HN-2 isolated from arsenic-contaminated soil in Hunan, China

Bioremediation of Water: A Sustainable Approach

Arsenic Removal and Biotransformation Potential of Exiguobacterium Isolated From an Arsenic‐Rich Soil of Chhattisgarh, India

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