Lipids extracted from Helicobacter pylori were separated into lipid classes by thin-layer chromatography. Simple H. pylori lipids consisted of cholesterol esters, triglycerides, free fatty acids, cholesterol, diacylglycerols, and monoacylglycerols. Fatty acids were released from each lipid class by acid methanolysis, and analyzed by gas liquid chromatography and mass spectrometry. Unique methoxy fatty acids, including 11-methoxy heptadecanoic and 11-methoxy nonadecanoic acids, were the major components of the cholesterol esters and triglycerides. The predominance of methoxy fatty acids in the cholesterol esters of H. pylori may contribute to the acid-resistant characteristic of this bacillus.
Abstract:Fatty acids were released from the total lipids of Helicobacter pylori by acid methanolysis and analyzed by gas liquid chromatography and mass spectrometry. Eight peaks of the fatty acid methyl esters were detected and identified to be tetradecanoic, hexadecanoic, octadecanoic, octadecenoic, octadecadienoic, cyclo [11, 12] methylene octadecanoic, 11-methoxy-heptadecanoic and 11-methoxy-nonadecanoic fatty acids. A small amount of hydroxy docosanoic acid was also detected after trimethylsilylation. Two fatty acids, 11-methoxy-heptadecanoic and 11-methoxy-nonadecanoic acids, are unique and the occurrence of these fatty acids in bacteria as well as Helicobacter pylori is reported for the first time.
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