Lipid composition and fatty acid analysis ofHelicobacter pylori

Inamoto, Yoshihito; Hamanaka, Sumiko; Hamanaka, Yuichiro; Nagate, Takatoshi; Kondo, Isamu; Takemoto, Tadayoshi; Okita, Kiwamu

doi:10.1007/bf02347505

Cited by 17 publications

(13 citation statements)

References 17 publications

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“…Mid-chain methoxylated acids have already been reported in non-marine bacteria (Inamoto et al 1995). The series of methoxylated FAMES reported in a Sicilian red algae showed similarities with the series identified in the present work, and the authors also confided to a genuine bacterial origin of methoxylated FAMEs (Barnathan et al 1998).…”

Section: Discussionsupporting

confidence: 70%

“…The m/z values in bold correspond to the fragments of the dominant isomers in bacterial phospholipids, whose spectra and fragmentation are presented in Figure 1. Loui and Moore (1979); 4, Kerger et al (1986); 5, Gerwick et al (1987); 6, Orgambide et al (1993); 7, Inamoto et al (1995); 8, Barnathan et al 1998;9, Mesguishe et al 1999;10, Drouin et al 2000. source of essential FAMEs in the microbial loop (Zhukova and Kharlamento 1999).…”

Section: Molecular Patterns Of Flagellate Fatty Acidsmentioning

confidence: 95%

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Polar lipid biomarkers of free-living bacteria from oligotrophic marine waters

2005

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Free-living marine bacteria isolated from oligotrophic Mediterranean waters were enriched in culture to characterize their phospholipid fatty acids (PLFAs). Odd chain iso-and anteisoFAMEs and nÀ16:0 were the predominant structural PLFAs, together with a homologous series identified as mid-chain methoxy FAMEs. The dominant methoxy fatty acids identified were 9-CH 3 O-15:0, 9-CH 3 -16:0 and 11-CH 3 O-17:0, occuring as pairs of stereoimers. Methoxy fatty acids accounted for up to 37% of PLFAs of free-living bacteria, which sets them as promising new biomarkers for bacteria of oligotrophic waters. Although similar homologues have already been characterized in a variety of eukaryotes and prokaryotes, methoxy fatty acids are identified here for the first time in marine bacteria. Analytical difficulties that may hinder the characterization of these biomarkers are presented, and structural elucidation keys by gas chromotography coupled to mass spectrometry are discussed. Whilst bacterial branched fatty acids were transferred to storage lipids of bacterivorous flagellates methoxy acids were not transferred to higher trophic levels in the studied conditions.

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Section: Discussionsupporting

confidence: 70%

Section: Molecular Patterns Of Flagellate Fatty Acidsmentioning

confidence: 95%

Polar lipid biomarkers of free-living bacteria from oligotrophic marine waters

2005

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“…pylori rapidly takes up cholesterol (3), a compound in serum, blood, and eucaryotic cell membranes. Interestingly, this cholesterol is glycosylated and comprises about 25% of the lipid membrane content of H. pylori (8,10,12), a unique feature among procaryotes. Since H. pylori does not appear to carry cholesterol biosynthesis genes in the genome (2,29), H. pylori must obtain the cholesterol from the host gastric mucosa in vivo.…”

Section: Discussionmentioning

confidence: 99%

Helicobacter pylori Growth and Urease Detection in the Chemically Defined Medium Ham's F-12 Nutrient Mixture

2001

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Obstacles continue to hinder in vitro studies of the gastric human pathogen Helicobacter pylori, including difficulty culturing the organism in the absence of serum or blood, rapid loss of viability following exponential growth due to autolysis, and the necessity for using high starting inocula. We demonstrate that H. pylori grows in the chemically defined broth medium Ham's F-12 nutrient mixture (F-12) in the absence of fetal bovine serum (FBS); this represents a breakthrough for studies in which serum components or proteins interfere with interpretation of results. Cultures can be continually passaged in fresh, FBS-free F-12 medium at an initial inoculum of only ϳ10 3 CFU/ml. All H. pylori strains (n ‫؍‬ 21), including fresh clinical isolates, grew in serum-free F-12. H. pylori grew poorly in the related medium, F-10, unless additional zinc was supplied. Enhanced growth of H. pylori in F-12 broth was obtained by addition of bovine serum albumin (BSA) (1 mg/ml), ␤-cyclodextrin (200 g/ml), or cholesterol (50 g/ml). H. pylori also grew in several simplified versions of F-12 broth lacking glucose and most vitamins but containing hypoxanthine, pyruvate, and all 20 amino acids. On F-12 medium solidified with agar, H. pylori only grew when BSA (98% pure; 1 mg/ml), cholesterol (50 g/ml), ␤-cyclodextrin (200 g/ml), or FBS (2 to 4%) was added; addition of urea and phenol allowed colorimetric detection of urease activity. Thus, F-12 agar plus cholesterol or ␤-cyclodextrin represents the first transparent chemically defined agar and the first urease indicator agar for H. pylori. Several lines of evidence suggested that BSA itself is not responsible for H. pylori growth enhancement in F-12 containing BSA or FBS. Taken together, these innovations represent significant advances in the cultivation and recovery of H. pylori using chemically defined media. Use of F-12 or its derivatives may lead to improved understanding of H. pylori metabolism, virulence factors, and transmission, and result in improved recovery and identification of H. pylori from clinical specimens.

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“…A representative Helicobacter species H. pylori is known to incorporate exogenous cholesterol into the bacterial cells [12][13][14][15][16][17][18][19]. This study demonstrated that the cells of H. felis and H. cinaedi absorb cholesterol added to the medium, as observed in H. pylori cells.…”

Section: Discussionmentioning

confidence: 66%

Phosphatidylethanolamine, but not Phosphatidylglycerol‐Cardiolipin, Isolated from Two Species of Helicobacter Binds Cholesterol over Cholesteryl Ester

Amgalanbaatar

Hosoda²,

Shimomura

2015

Lipids

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This study demonstrated that the cells of Helicobacter felis and Helicobacter cinaedi spontaneously absorb cholesterol added to the medium. A recent study by our group has revealed that phosphatidylethanolamine (PtdEtn) of Helicobacter pylori contains myristic acid as the most predominant saturated fatty acid and that the PtdEtn of this bacterium binds cholesterol more selectively than cholesteryl ester. We, therefore, isolated the PtdEtn from the two Helicobacter species to analyze the hydrophobic interaction between cholesterol and its glycerophospholipid. PtdEtn of the Helicobacter bacteria interacted more selectively with cholesterol than with cholesteryl ester, and the degree of the selective binding of cholesterol was higher in the PtdEtn than in the phosphatidylglycerol-cardiolipin of the same bacteria. These results suggest the possibility that the cells of H. felis and H. cinaedi may contain abundant PtdEtn with myristic acid. On this basis, we analyzed the PtdEtn molecular species of the Helicobacter bacteria and demonstrated that the PtdEtn containing myristic acid accounts for more than 35% in the total PtdEtn. These results suggest that the myristoyl PtdEtn takes part in the absorption of cholesterol in H. felis and H. cinaedi.

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Lipid composition and fatty acid analysis ofHelicobacter pylori

Cited by 17 publications

References 17 publications

Polar lipid biomarkers of free-living bacteria from oligotrophic marine waters

Polar lipid biomarkers of free-living bacteria from oligotrophic marine waters

Helicobacter pylori Growth and Urease Detection in the Chemically Defined Medium Ham's F-12 Nutrient Mixture

Phosphatidylethanolamine, but not Phosphatidylglycerol‐Cardiolipin, Isolated from Two Species of Helicobacter Binds Cholesterol over Cholesteryl Ester

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