This work reports a novel wireless microfluidic biosensor based on low temperature co-fired ceramic (LTCC) technology. The wireless biosensor consists of a planar spiral inductor and parallel plate capacitor (LC) resonant antenna, which integrates with microchannel bends in the LTCC substrate. The wireless response of the biosensor was associated to the changes of its resonant frequency due to the alteration in the permittivity of the liquid flow in the microchannel. The wireless sensing performance to different organic liquids with permittivity from 3 to 78.5 was presented. The measured results are in good agreement with the theoretical calculation. The wireless detection for the concentration of glucose in water solution was investigated, and an excellent linear response and repeatability were obtained. This kind of LC wireless microfluidic sensor is very promising in establishing wireless lab-on-a-chip for biomedical and chemical applications.
In this study, we described in detail a combinatorial
enzymatic
synthesis approach to produce a series of unnatural long-chain β-branch
pyrones. We attempted to investigate the catalytic potential of a
highly promiscuous enzyme type III PKS to catalyze the non-decarboxylative
condensation reaction by two molecules of fatty acyl diketide-N-acetylcysteines (diketide-NACs) units. Two non-natural
long-chain (C16, C18) fatty acyl diketide-NACs
were prepared successfully for testing the ability of non-decarboxylative
condensation. In vitro, 12 novel naturally unavailable long-chain
β-branch pyrones were generated by one-pot formation and characterized
by ultraviolet–visible spectroscopy and high-resolution liquid
chromatography–mass spectrometry. Interestingly, enzymatic
kinetics result displays that this enzyme exhibits the remarkable
compatibility to various non-natural long-chain substrates. These
results would be useful to deeply understand the catalytic mechanism
of this enzyme and further extend the application of enzymatic synthesis
of non-natural products.
Three kinds of borosilicate glass-ceramics Low temperature co-fired ceramics (LTCC) substrate materials, CaB -Si-O (CBS), Ca-Al-B-Si-O (CABS), and Ca-Mg-B-Si-O (CMBS), have been prepared, and the biocompatibility of these materials was evaluated. Compared with CBS and CMBS samples, the CABS sample presented the lowest crystallinity and dissolution in the bioleaching experiments. The direct and indirect cell culturing results showed that the CABS sample also possessed the best biocompatibility to the bone mesenchymal stem cells (BMSCs) due to its good bio-stability and surface micro-pore structure. The effects of surface topography and released ions on cytocompatibility were analyzed. These results were expected to offer some guideline information for developing LTCC substrate materials used in the microfluidic biosensors for the future biological and medical application. K E Y W O R D S borosilicate glass, dielectric materials/properties, glass-ceramics T A B L E 2 Direct cell culturing results based on the CCK-8 assay Samples RGR % (mean ± SD)
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.