Biocompatibility of borosilicate glass‐ceramics based LTCC materials for microfluidic biosensor application

Abstract

Three kinds of borosilicate glass-ceramics Low temperature co-fired ceramics (LTCC) substrate materials, CaB -Si-O (CBS), Ca-Al-B-Si-O (CABS), and Ca-Mg-B-Si-O (CMBS), have been prepared, and the biocompatibility of these materials was evaluated. Compared with CBS and CMBS samples, the CABS sample presented the lowest crystallinity and dissolution in the bioleaching experiments. The direct and indirect cell culturing results showed that the CABS sample also possessed the best biocompatibility to the bone mesenchymal stem cells (BMSCs) due to its good bio-stability and surface micro-pore structure. The effects of surface topography and released ions on cytocompatibility were analyzed. These results were expected to offer some guideline information for developing LTCC substrate materials used in the microfluidic biosensors for the future biological and medical application. K E Y W O R D S borosilicate glass, dielectric materials/properties, glass-ceramics T A B L E 2 Direct cell culturing results based on the CCK-8 assay Samples RGR % (mean ± SD)

Perfused Platforms to Mimic Bone Microenvironment at the Macro/Milli/Microscale: Pros and Cons

“…By further analyzing the limitations of microfluidics, the standard materials used as ECM in these devices are thermoresponsive organic hydrogels (matrigel, collagen, fibrin) that can mimic or not the organic phase of bone and are easily injectable, but do not resemble bone inorganic composition and microarchitecture. Recent studies have paved the way to functionalize hydrogels with an inorganic phase ( Liang et al, 2020 ), such as calcium phosphate (HA microbeads, HA nanoparticles, tricalcium phosphate TCP), borosilicate glass-ceramics, that, however, are still far from reproducing bone tissue characteristics ( Sharma et al, 2021 ). Moreover, in micro-bioreactor, cell culture surface is very small (around 0.5–0.8 mm 2 ) and can be seeded only with a few thousand cells.…”