Background and Objectives The efficacy and outcomes of aspirin in local defects and the use of platelet‐rich fibrin (PRF) in periodontal defects were investigated. Whether the PRF/aspirin complex is a suitable scaffold and delivery system to carry sustained‐release aspirin/salicylic acid to promote periodontal bone regeneration was determined. Material and Methods PRF and PRF/aspirin complex were prepared. The concentrations of aspirin/salicylic acid released from the PRF/aspirin complex were calculated at 37°C. Periodontal ligament mesenchymal cells were cultured on six‐well plates with PRF or PRF/aspirin complex gel to analyze proliferation and migration. The alveolar bone between the inferior buccal mesial root and anterior buccal distal root of the first maxillary molar was removed in 15 rats randomly divided into three groups: no treatment, PRF or PRF/aspirin complex. Twelve weeks post‐transplantation, 2D/3D micro‐computed tomography and histomorphometric technique were used for quantitative analyses. Results The PRF/aspirin complex provided a sustained‐release aspirin/salicylic acid. Peak concentrations occurred 4 hours after transplantation and were sustained to 48 hours at 37°C; the total concentration of released aspirin/salicylic acid was 83.5 mg/mL, respectively. The sustained‐release promoted the proliferation and migration of periodontal ligament mesenchymal cells. Micro‐computed tomography and histological data showed that both the PRF and PRF/aspirin complex enhanced periodontal bone formation (P<.05). Moreover, the new bone formation was two times greater in the PRF/aspirin complex group than the PRF group. Conclusion Aspirin/salicylic acid could be sustained‐released from PRF/aspirin complex, which could inhibit inflammation and improve the function of mesenchymal cells. The data might provide a new safe and easy clinical therapeutic strategy to promote periodontal bone reparation.
Background: Autologous fat grafting is an efficient procedure in plastic surgery. However, its long-term tissue absorption is variable and technique-dependent. Platelet-rich plasma positively affects fat-grafting outcomes but still has shortcomings, and platelet-rich fibrin has been reported to have efficacy in fat transplantation. Here, we compared the effects of platelet-rich fibrin and platelet-rich plasma in fat grafting using histologic analysis. Methods: Twenty rabbits were divided randomly into two groups. In each group, the groin region fat pads were cut into 1-mm3 granules. Platelet-rich fibrin–treated or platelet-rich plasma–treated fat granules were transplanted into one ear, whereas the contralateral ear was transplanted with normal saline–treated fat granules. Histologic characteristics and capillary density of grafted tissue were analyzed 12 weeks after fat grafting. Results: The grafted fat in the platelet-rich fibrin–treated group showed higher tissue retention than that in the control group [weight retention, 19.57 percent (interquartile range, 13.87 to 29.93 percent) versus 9.04 percent (interquartile range, 6.16 to 16.80 percent), p < 0.05; and volume retention, 18.00 percent (interquartile range, 10.50 to 26.50 percent) versus 8.00 percent (interquartile range, 5.75 to 13.25 percent), p < 0.05] and higher neovascularized capillary density than that in the platelet-rich plasma–treated and control groups. The platelet-rich plasma–treated group showed higher vessel density without superior tissue retention compared with the control group. Conclusion: Platelet-rich fibrin increased tissue retention, quality, and vascularization of grafted fat compared with the control group and showed effects similar to those of platelet-rich plasma on tissue retention and histologic graft improvement.
Purpose As a key part of the algae removal equipment in the middle route of South-to-North Water Diversion Project, the static analysis and dynamic analysis are carried out for the structural characteristics of the frame. The paper aims to discuss this issue. Design/methodology/approach First, the model is constructed for analysis. Second, finite element analysis are carried out. And finally, test designed is used for the construction. Findings The optimization scheme that minimizes the quality under the condition of satisfying the allowable stress is found, and the quality is reduced by 6.88 percent. Originality/value The paper is based on the occurrence of seasonal algae in the main channel of the Middle Route of the South-to-North Water Diversion Project, an automatic algae-removing equipment was designed.
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