Platelet-Rich Plasma and Platelet-Rich Fibrin Enhance the Outcomes of Fat Grafting: A Comparative Study

Abstract

Background: Autologous fat grafting is an efficient procedure in plastic surgery. However, its long-term tissue absorption is variable and technique-dependent. Platelet-rich plasma positively affects fat-grafting outcomes but still has shortcomings, and platelet-rich fibrin has been reported to have efficacy in fat transplantation. Here, we compared the effects of platelet-rich fibrin and platelet-rich plasma in fat grafting using histologic analysis. Methods: Twenty rabbits were divided randomly into two groups. In each group, the groin region fat pads were cut into 1-mm3 granules. Platelet-rich fibrin–treated or platelet-rich plasma–treated fat granules were transplanted into one ear, whereas the contralateral ear was transplanted with normal saline–treated fat granules. Histologic characteristics and capillary density of grafted tissue were analyzed 12 weeks after fat grafting. Results: The grafted fat in the platelet-rich fibrin–treated group showed higher tissue retention than that in the control group [weight retention, 19.57 percent (interquartile range, 13.87 to 29.93 percent) versus 9.04 percent (interquartile range, 6.16 to 16.80 percent), p < 0.05; and volume retention, 18.00 percent (interquartile range, 10.50 to 26.50 percent) versus 8.00 percent (interquartile range, 5.75 to 13.25 percent), p < 0.05] and higher neovascularized capillary density than that in the platelet-rich plasma–treated and control groups. The platelet-rich plasma–treated group showed higher vessel density without superior tissue retention compared with the control group. Conclusion: Platelet-rich fibrin increased tissue retention, quality, and vascularization of grafted fat compared with the control group and showed effects similar to those of platelet-rich plasma on tissue retention and histologic graft improvement.

Fechamento cirúrgico de comunicação buco-sinusal com uso de L-PRF: um relato de caso

“…No presente relato de caso a utilização de L-PRF associado a um retalho vestibular sem interposição do corpo adiposo da bochecha resultou em regeneração óssea adequada e cicatrização saudável da mucosa, sem a ocorrência de complicações pós-operatórias. Portanto, essa técnica pode ser considerada viável e uma alternativa ao retalho pediculado com o corpo adiposo da bochecha, que apesar da ótima taxa de sucesso, pode resultar em alterações do contorno facial, hematoma e lesões nervosas, além de inviabilizar a possibilidade de sua utilização em uma eventual situação futura (Gheisari et al 2019;Nair et al 2019;Xiong et al 2019).…”

Current art of combination therapy with autologous platelet‐rich plasma for stable vitiligo: A meta‐analysis

“…Activated platelets can release autogenous growth factors that may initiate signalling cascades and lead to multiple intracellular changes, promoting the proliferation, migration and differentiation of stem cells and regulating local inflammation and immune responses 5,6 . Due to its healing and regenerative properties, PRP has been widely applied in the aesthetic and dermatology fields, involving facial rejuvenation, androgenic alopecia enrichment, chronic hard‐to‐heal skin ulcers healing, acne scars improvement, fat grafting enrichment, vitiligo, and melisma treatment as well 7‐11 . Some studies also suggested that PRP could stimulate the proliferation of keratinocyte and fibroblasts, enhance interaction of keratinocyte and fibroblasts with melanocytes, and inhibit apoptosis of melanocytes 12‐14 .…”

Electrospinning With Lyophilized Platelet-Rich Fibrin Has the Potential to Enhance the Proliferation and Osteogenesis of MC3T3-E1 Cells

“…Platelet-rich fibrin (PRF) is a second-generation platelet concentrate to replace platelet-rich plasma (PRP), and has become an attractive strategy in tissue grafting and regenerative medicine because it has abundant tissue healing-promoting cytokines and interleukins (ILs), including transforming growth factor beta 1 (TGF-β1), basic fibroblast growth factor (bFGF), platelet derived growth factor (PDGF), vascular endothelial growth factor (VEGF), IL-6, IL-8, IL-11, and so on (Masuki et al, 2016). Furthermore, PRF has been verified to increase tissue retention, quality, and neovascularized capillary density of grafted fat (Xiong et al, 2019). In addition, PRF has also been demonstrated to facilitate the regeneration of bone, periodontal tissue, and dental pulp (Chang and Zhao, 2011;Ji et al, 2015;You et al, 2019).…”