A diagnosis of heavy metal poisoning in sheep living on pastures in the vicinity of a smelting facility in the Wumeng mountain area of China was based on laboratory tests and clinical symptoms. Furthermore, heavy metal contamination in the food chain was found to have a deleterious effect on the health of local residents. The levels of copper(Cu), zinc(Zn), cadmium (Cd), and lead (Pb) in irrigation water, soil, forages, and animal tissues were measured in samples taken from the vicinity of a smelting facility and control samples. Heavy metal contents in food (corn, rice, and wheat), as well as in human tissues (blood and hair) obtained from local residents were also determined. Hematological values were also determined in human and animal samples. The content of Cu, Zn, Cd, and Pb in irrigation water, soils, and forages were markedly higher in affected areas than in samples from healthy pastures. Concentrations of Cd and Pb were 177.82 and 16.61 times greater in forages than controls, respectively, and 68.71 and 15.66 times greater in soils than controls, respectively. The heavy metal content in food (corn, rice, and wheat) from affected areas was markedly higher than in the control samples. Cd and Pb content in the tissues of affected sheep were markedly higher than in control animals (P < 0.01), while concentrations of Cd and Pb in blood and hair samples from local residents were markedly higher than in control samples (P < 0.01). The occurrence of anemia in affected humans and animals followed a hypochromic and microcytic pattern. The intake of Cd and Pb was estimated according to herbage ingestion rates. It was found that the levels of Cd and Pb which accumulated in sheep through the ingestion of vegetation growing in the sites closest to the smelter were approximately 3.36 and 38.47 mg/kg body wt./day, respectively. Such levels surpassed the fatal dosages for sheep of 1.13 mg Cd/kg body wt/day and 4.42 mg Pb/kg body wt./day. The serum total antioxidant capacity in affected humans and animals was significantly lower than in the controls (P < 0.01). Serum protein parameters in affected humans and animals were significantly reduced (P < 0.01); therefore, it was concluded that heavy metal contamination caused harm to sheep, and also posed a significant risk to humans living in the vicinity of the zinc smelting facility.

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The study of soil ecological stoichiometry has mainly focused on surface soils. Exploring the nutrient content and stoichiometric characteristics of deep soils in karst areas helps us to understand the intrinsic relation mechanism and provides scientific evidence for making full use of fractured soil. We analyzed soil nutrient content, nutrient supply intensity, stoichiometric ratios, and their interrelations at different soil depths in a karst plateau mountain area of China. Soil nutrient supply capacity was relatively low in the study area. The nitrogen and potassium supply capacity was highest in the surface layer, while phosphorus supply capacity was highest in deep soil. Carbon, nitrogen, phosphorus and potassium made varying contributions to the ecological stoichiometric ratios. Soil nitrogen and phosphorus were found to be deficient in the karst plateau mountain area of China. There was certain coordination between nutrient supply intensity and ecological stoichiometric ratios.

Soil bacteria play an important role in regulating the process of vegetation restoration in karst ecosystems. However, the effects of vegetation restoration for different cultivated pastures on soil bacterial communities in the karst rocky desertification regions remain unclear. Therefore, we hypothesized that mixed pasture is the most effective for soil bacterial communities among different vegetation restorations. In this study, we systematically studied the soil properties and soil bacterial communities in four vegetation restoration modes [i.e., Dactylis glomerata pasture (DG), Lolium perenne pasture (LP), Lolium perenne + Trifolium repens mixed pasture (LT), and natural grassland (NG)] by using 16S rDNA Illumina sequencing, combined with six soil indicators and data models. We found that the vegetation restoration of cultivated pastures can improve the soil nutrient content compared with the natural grassland, especially LT treatment. LT treatment significantly increased the MBC content and Shannon index. The vegetation restoration of cultivated pastures significantly increased the relative abundance of Proteobacteria, but LT treatment significantly decreased the relative abundance of Acidobacteria. Soil pH and MBC significantly correlated with the alpha diversity of soil bacterial. Soil pH and SOC were the main factors that can affect the soil bacterial community. FAPROTAX analysis showed LT treatment significantly decreased the relative abundance of aerobic chemoheterotrophs. The results showed that the bacterial communities were highly beneficial to soil restoration in the LT treatment, and it confirmed our hypothesis. This finding provides a scientific reference for the restoration of degraded ecosystems in karst rocky desertification areas.