iTRAQ-based proteomic analysis to identify molecular mechanisms of the selenium deficiency response in the Przewalski's gazelle

Shen, Xiaoyun; Huo, Bin; Wu, Ting; Song, Chunjie; Chi, Yongkuan

doi:10.1016/j.jprot.2019.103389

Cited by 44 publications

(20 citation statements)

References 51 publications

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“…For instance, label-free proteomics showed selenoprotein P as the most abundant proteins in milk of cows, more precisely, in cows producing milk with A2A2-β-casein variants [86]. The protocols can be quantitative by using isobaric tagging for relative and absolute Quantification (iTRAQ) [80,[87][88][89].…”

Section: Electrospray Msmentioning

confidence: 99%

Characterization and Quantification of Selenoprotein P: Challenges to Mass Spectrometry

Lamarche

Ronga

Szpunar

et al. 2021

IJMS

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Selenoprotein P (SELENOP) is an emerging marker of the nutritional status of selenium and of various diseases, however, its chemical characteristics still need to be investigated and methods for its accurate quantitation improved. SELENOP is unique among selenoproteins, as it contains multiple genetically encoded SeCys residues, whereas all the other characterized selenoproteins contain just one. SELENOP occurs in the form of multiple isoforms, truncated species and post-translationally modified variants which are relatively poorly characterized. The accurate quantification of SELENOP is contingent on the availability of specific primary standards and reference methods. Before recombinant SELENOP becomes available to be used as a primary standard, careful investigation of the characteristics of the SELENOP measured by electrospray MS and strict control of the recoveries at the various steps of the analytical procedures are strongly recommended. This review critically discusses the state-of-the-art of analytical approaches to the characterization and quantification of SELENOP. While immunoassays remain the standard for the determination of human and animal health status, because of their speed and simplicity, mass spectrometry techniques offer many attractive and complementary features that are highlighted and critically evaluated.

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Section: Electrospray Msmentioning

confidence: 99%

Characterization and Quantification of Selenoprotein P: Challenges to Mass Spectrometry

Lamarche

Ronga

Szpunar

et al. 2021

IJMS

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“…The enzymatic system consists of antioxidant enzymes, including mainly CAT, SOD and GSH-Px. Antioxidant system can catalyze rapidly the superoxide anion (O 2-) to produce disproportionation reaction, eliminate superoxide anion, and protect cells of organism from damage of free radicals [15][16][17][18]. Cu supplementation, in the range of growth promotion, can increase activities of GSH-Px, SOD and CP in serum in cattle, pigs and chickens [17].…”

Section: Introductionmentioning

confidence: 99%

Effect of Different Copper Levels in Feed on Antioxidant Capacity in Stocking the Native Sheep in the Wumeng Prairie

Wu¹,

Xiong²,

Jian³

2021

Pol. J. Environ. Stud.

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The purpose of this research is to better understand the effect of different copper (Cu) levels of feed on the antioxidant capacity in the native sheep, solve the adverse effects of local seasonal Cu deficiency on sheep, and provide a reasonable reference for Cu supplementation. 10 healthy native sheep were selected as the control group (C group). 40 Cu-deficient native sheep were selected as tested group, and divided randomly into 4 groups (n = 10), supplied by CuSO 4 (0, 20, 40, 60 mg/kg for group I, group II, group III and group IV, respectively). The results showed that the level of Cu, hemoglobin (Hb), packed-cell volume (PCV), albumin (ALB), the activities of ceruloplasmin (CP), Cu/Znsuperoxide dismutase (Cu/Zn-SOD) and catalase (CAT) in group I were significantly lower than those in C group, group II, III, IV (P<0.05), but malondialdehyde (MDA) concentration was higher. The activities of these antioxidant enzymes and MDA concentration had no significant difference between C group, group II, III, IV (P>0.05), but group III had the highest antioxidant enzymes activity and the lowest MDA concentration. There was no significant difference in manganese (Mn), zinc (Zn), iron (Fe), molybdenum (Mo), selenium (Se), red blood cell count (RBC), white blood cell count (WBC), blood urea nitrogen (BUN), alanine transaminase (ALT), alkaline phosphatase (ALP), aspartate aminotransferase (GOT), total cholesterol (TC), manganese-superoxide dismutase (Mn-SOD) between all groups (P>0.05). Conclusion: The function of antioxidant system of the native sheep in Weining County have seriously reduced. Added CuSO 4 to the diet increase the activity of antioxidant enzymes and improve the health parameters, but them did not increase linearly. Added 40 mg/kg CuSO 4 , the activity of antioxidant enzymes was the highest and the content of MDA was the lowest. Therefore, it is recommended to add 40 mg/kg CuSO 4 to diet of the native sheep in Weining County.

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“…Mineral elements are key nutrients for animals and humans [5,14]. Too high contents of mineral elements in soil and herbage will cause mineral poisoning in animals [15]. Therefore, the study on the characteristics of mineral elements in soil, herbage, and livestock can not only clarify the distribution law of minerals in pasture, but also help to understand status of nutrition in grazing animals [16,17].…”

Section: Effects Of S Fertilization On Mineral Elements In Herbagementioning

confidence: 99%

Effects of Sulfur Fertilization on Antioxidant Capacity of Wumeng Semi-Fine Wool Sheep in the Wumeng Prairie

Li¹,

Wang²,

Shen³

2021

Pol. J. Environ. Stud.

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To assess the impact of sulfur(S) fertilization on antioxidant capacity of grazing Wumeng semifine wool sheep in copper(Cu) polluted meadow, and explore the control methods of Cu pollution in natural pasture, fertilizer treatment and grazing experiments were carried out in Weining County of the Wumeng Prairie, Guizhou Province, Southwest China. 24 ha Cu-polluted meadows were fenced, and randomly divided into four groups (3 replications/group, 2 ha/replication): 1) control group, no fertilizer; 2) the tested groups, applied ammonium sulfate [(NH4) 2 SO 4 ] (300, 400, and 500 kg/ha for group I, group II, and group III, respectively). A total of 72 healthy Wumeng semi-fine wool sheep (aged one year, 32.8±1.3 kg) were used in this study. All animals were randomly divided into 4 groups (3 replications/group, 6 sheep/replication) and assigned to the tested pastures. The grazing experiment lasted for 60 days. The results showed that the contents of N, Mn, Zn, and S in herbage in fertilized pastures were markedly higher than those in control group (P<0.01). The contents of Cu, Fe, and Se in herbage in fertilized pastures were significantly lower than those in control group (P<0.01). There were no extreme differences among the fertilized pastures (P>0.05). The contents of Mn, Zn, and S in serum and liver in the tested sheep were significantly higher than those in control animals (P<0.01). The contents of Cu, Fe, and Se in serum and liver in the tested sheep were extremely lower than those in control animals (P<0.01). The levels of Hb, RBC, and PCV in blood in the tested sheep, and the activities of serum SOD, GSH-Px, T-AOC, CAT, and Cp in the tested sheep were extremely higher than those in control sheep (P<0.01). The contents of serum MDA in the tested sheep were significantly lower than those in control animals (P<0.01). There were no great differences among the tested groups in physiological and biochemical parameters (P>0.05). The current results indicated that fertilization of

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iTRAQ-based proteomic analysis to identify molecular mechanisms of the selenium deficiency response in the Przewalski's gazelle

Cited by 44 publications

References 51 publications

Characterization and Quantification of Selenoprotein P: Challenges to Mass Spectrometry

Characterization and Quantification of Selenoprotein P: Challenges to Mass Spectrometry

Effect of Different Copper Levels in Feed on Antioxidant Capacity in Stocking the Native Sheep in the Wumeng Prairie

Effects of Sulfur Fertilization on Antioxidant Capacity of Wumeng Semi-Fine Wool Sheep in the Wumeng Prairie

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