Effect of Different Copper Levels in Feed on Antioxidant Capacity in Stocking the Native Sheep in the Wumeng Prairie

Abstract

The purpose of this research is to better understand the effect of different copper (Cu) levels of feed on the antioxidant capacity in the native sheep, solve the adverse effects of local seasonal Cu deficiency on sheep, and provide a reasonable reference for Cu supplementation. 10 healthy native sheep were selected as the control group (C group). 40 Cu-deficient native sheep were selected as tested group, and divided randomly into 4 groups (n = 10), supplied by CuSO 4 (0, 20, 40, 60 mg/kg for group I, group II, group III and group IV, respectively). The results showed that the level of Cu, hemoglobin (Hb), packed-cell volume (PCV), albumin (ALB), the activities of ceruloplasmin (CP), Cu/Znsuperoxide dismutase (Cu/Zn-SOD) and catalase (CAT) in group I were significantly lower than those in C group, group II, III, IV (P<0.05), but malondialdehyde (MDA) concentration was higher. The activities of these antioxidant enzymes and MDA concentration had no significant difference between C group, group II, III, IV (P>0.05), but group III had the highest antioxidant enzymes activity and the lowest MDA concentration. There was no significant difference in manganese (Mn), zinc (Zn), iron (Fe), molybdenum (Mo), selenium (Se), red blood cell count (RBC), white blood cell count (WBC), blood urea nitrogen (BUN), alanine transaminase (ALT), alkaline phosphatase (ALP), aspartate aminotransferase (GOT), total cholesterol (TC), manganese-superoxide dismutase (Mn-SOD) between all groups (P>0.05). Conclusion: The function of antioxidant system of the native sheep in Weining County have seriously reduced. Added CuSO 4 to the diet increase the activity of antioxidant enzymes and improve the health parameters, but them did not increase linearly. Added 40 mg/kg CuSO 4 , the activity of antioxidant enzymes was the highest and the content of MDA was the lowest. Therefore, it is recommended to add 40 mg/kg CuSO 4 to diet of the native sheep in Weining County.

Gene Expression of Oxidative/Antioxidative Markers, VDR, CAMK4 and Ceruloplasmin in Baladi Sheep a with Minerals Deficiency

“…Monitoring the oxidant/antioxidant status in the diseased animal revealed a significant increase in serum concentration of MDA with significant decrease in serum enzymatic activities of TAC, GSH-Px, CAT, SOD and Cp. These results were in line with those of [13], who found that a lower intake of Cu in the diet was linked to lower levels of GPx, SOD, and CAT and higher levels of MDA, as well as a decrease in the body's ability to create antioxidants. Conversely, increasing the amount of copper in the diet boosts the body's antioxidant capability.…”

“…SOD is a crucial part of the antioxidant system because it scavenges free radicals in animals, which allows it to oppose and prevent the harm that free radicals do to cells while also repairing the damage over time [45]. Since Cu/Zn-SOD is the most prevalent of the four types of SOD, improving Cu/Zn-SOD production and synthesis can be achieved by raising the level of Cu in vivo [17] [13]. The reaction product of SOD is the substrate of GSH-Px and CAT [17].…”

“…The component of the antioxidant system, which protects an organism from free radicals, consists of both enzymatic and non-enzymatic mechanisms [12]; [13] . The antioxidants enzymes that comprise the enzymatic system are mostly glutathione peroxidase GSH-Px, superoxide dismutase (SOD) and catalase (CAT).…”

“…The antioxidants enzymes that comprise the enzymatic system are mostly glutathione peroxidase GSH-Px, superoxide dismutase (SOD) and catalase (CAT). Additionally, the primary components of the non-enzymatic system are vitamin, cysteine, glutathione, and mineral elements [13]. Zinc, copper and selenium elements are used in the creation of antioxidant enzymes [9].…”