The three-dimensional structure and
the surface wettability of
tissue engineering scaffolds are the crucial factors in the process
of tissue regeneration. In this study, biodegradable poly(ester-ether-urethane)ureas
(PEEUUs) were synthesized by employing polycaprolactone diol (PCL
diol) and polyethylene glycol (PEG) with the settled molar ratios
100:0, 85:15, 65:35, and 50:50 as the primary monomer. Then, the synthesized
PEEUUs were processed into a three-dimensional microporous spongy
scaffold (3DMSS) through the thermally induced phase separation technology.
The results showed that the content of PEG in the primary monomer
has a significant impact on the microstructure, porosity, wettability,
water absorption, biodegradability rate, and mechanical properties
of the microporous spongy scaffold (MSS). Cell experiments in vitro
proved that the addition of PEG in the molecular chain of PEEUUs significantly
improves the ability of cell adhesion and proliferation of 3DMSS.
All the results demonstrated the better cell migration effect of 3DMSS
with PEG in injured tissue defect repair, indicating their great potential
for facilitating rapid reconstruction of a specific tissue in clinic.
Intractable
skin defects, which involve excessive inflammation
and bacterial infections, caused by burns, trauma, and diabetes are
a major challenge for clinicians. Compared with traditional skin transplantation,
tissue-engineered skin has the advantages of a wide range of sources,
prominent biological activity, and no damage to the donor area during
the operation. Therefore, an effective wound-healing mat with antibacterial,
anti-inflammatory, and microvascularization bioactivities is urgent
to be developed. In this study, we have synthesized a poly(ester-urethane)urea/silk
fibroin/magnolol nanofibrous composite mat (PSM) through electrospinning
and post-hydrogen bond cross-linking. The results show that the hybrid
magnolol has no adverse effect on the microstructure, porosity, wettability,
and mechanical properties of PSM. Antibacterial experiments and cytocompatibility
in vitro have proved that the addition of magnolol significantly improves
the antibacterial ability and promotes cell adhesion and proliferation
of PSM. In addition, the wound model of rat back and H&E staining,
Masson trichrome staining, and CD31 and CD68 immunofluorescence staining
were performed for evaluating the therapeutic efficiency of PSM. All
the results show that the better wound treatment effect of magnolol
hybrid nanofibrous mats in infectious skin tissue defected repair
indicates their great potential for wound healing clinically.
Wound repair remains a huge clinical challenge, which can cause bleeding, infection, and patient death. In our current research, a bioactive, injectable, multifunctional composite hydrogel doped with nanospheres was prepared with antibacterial and angiogenesis-promoting functions for the treatment of wounds. Amino groups in ε-polylysine (ε-EPL) undergo dynamic Schiff base reaction cross-linking with oxidized hyaluronic acid (OHA), and F127 exhibits unique temperature sensitivity to form an injectable thermosensitive hydrogel (FHE10), which can form a hydrogel to cover the wound at body temperature. Nanospheres (PNs) prepared using poly (glyceryl-sebacate-acrylate) (PGSA) were loaded into hydrogels (FHE10) for promoting wound repair. The prepared FHE10 exhibited rapid gelation, good injectable abilities, and showed resistance to the flourish of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). In vitro investigations showed that FHE10 had good hemocompatibility and cytocompatibility. FHE10@PNs exhibited good proliferation, migration, and tube formation of human umbilical vein endothelial cells (HUVECs) and human foreskin fibroblasts (HFF-1). Furthermore, FHE10@PNs significantly promoted reepithelialization and collagen deposition as well as micro-vascularization compared with the use of FHE10 or PNs alone, thereby accelerating the repair of wounds. In general, this study demonstrated that the multifunctional injectable composite hydrogel showed great potential in wound treatment.
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