We present the development of a client-side
web-based simulator
for complex electrophoresis phenomena, including isotachophoresis.
The simulation tool is called Client-based Application for Fast Electrophoresis
Simulation (CAFES). CAFES uses the broad cross-browser compatibility
of JavaScript to provide a rapid and easy-to-use tool for coupled
unsteady electromigration, diffusion, and equilibrium electrolyte
reactions among multiple weak electrolytes. The code uses a stationary
grid (for simplicity) and an adaptive time step to provide reliable
estimates of ion concentration dynamics (including pH profile evolution),
requiring no prior installation nor compilation. CAFES also offers
a large database of commonly used species and their relevant physicochemical
properties. We present a validation of predictions from CAFES by comparing
them to experimental data of peak- and plateau-mode isotachophoresis
experiments. The code yields accurate estimates of interface velocity,
plateau length and relative intensity, and pH variations while significantly
reducing the computation time compared to existing codes. The tool
is open-source and available for free at .
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