An efficient amperometric biosensor based on well-crystallized leaf-like CuO nanoparticles for detecting glucose has been proposed. The leaf-like CuO nanoparticles, synthesized by a simple one-step hydrothermal method, were characterized by X-ray diffraction (XRD), scanning electron microscope (SEM) and transmission electron microscopy (TEM) for the morphology study. Under the optimal condition, the electrochemical behaviour of the leaf-like CuO nanoparticles modified electrode for detection of glucose exhibited high sensitivity of 246 mA/mM/cm 2 , short response time (within 5 s), linear dynamic range from 1.0 to 170 mM (R 2 = 0.9995), and low limit of detection (LOD) (S/N = 3) of 0.91 mM. The high sensitivity, good reproducibility, stability, and fast amperometric sensing towards oxidation of glucose, make this biosensor promising for future application.
Hybrid nanocomposites of carboxyl-terminated generation 4 (G 4) poly(amidoamine) dendrimers (PAMAM) with gold nanoparticles (AuNPs) encapsulated inside them were synthesized under ultrasound irradiation. The obtained nanocomposites were used to fabricate highly sensitive amperometric glucose biosensor which exhibited a high and reproducible sensitivity of 2.9 mA/mM/cm(2), response time less than 5 s, linear dynamic range from 0.1 to 15.8 microM, correlation coefficient of R(2)=0.9988, and limit of detection (LOD), based on S/N ratio (S/N=3) of 0.05 microM. A value of 2.7 mM for the apparent Michaelis-Menten constant K(M)(app) was obtained. The high sensitivity, wider linear range, good reproducibility and stability make this biosensor a promising candidate for portable amperometric glucose biosensor.
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