Despite the known influence of nitrogen fertilization and groundwater conditions on soil microbial communities, the effects of their interactions on bacterial composition of denitrifier communities have been rarely quantified. Therefore, a large lysimeter experiment was conducted to examine how and to what extent groundwater table changes and reduced nitrogen application would influence the bacterial composition of nirK-type and nirS-type genes. The bacterial composition of nirK-type and nirS-type genes were compared at two levels of N input and three groundwater table levels. Our results demonstrated that depression of groundwater table, reduced nitrogen application and their interactions would lead to drastic shifts in the bacterial composition of nirS-type and nirK-type genes. Structural equation models (SEMs) indicated that depression of groundwater table and reduced nitrogen application not only directly altered the species composition of denitrifier bacterial communities, but also indirectly influenced them through regulating soil nutrient and salinity. Furthermore, the variation in soil NO3−–N and electrical conductivity caused by depression of groundwater table and reduced nitrogen application played the most important role in altering the community composition of denitrifier bacterial communities. Together, our findings provide first-hand evidence that depression of groundwater table and reduced nitrogen application jointly regulate the species composition of denitrifier bacterial communities in agricultural soil. We highlight that local environmental conditions such as groundwater table and soil attributes should be taken into account to enrich our knowledge of the impact of nitrogen fertilization on soil denitrifier bacterial communities, or even biogeochemical cycles.
The interest in reusing wastewater for irrigation is being popularized in most countries. The objective of this study was to evaluate the effects of different wastewater and nitrogen fertilizer on soil fertility and plant quality, as well as to identify the optimal irrigation mode in the North China Plain. A total of nine treatments, including control (groundwater, no fertilizer), piggery wastewater, reclaimed water, and saline water, combined with nitrogen fertilizer (300 kg/ha and 200 kg/ha), were conducted in a greenhouse in 2019 (Xinxiang, Henan Province). Soil pH, electrical conductivity, organic matter, heavy metals contents, and cucumber yield and quality were analyzed. The results showed that: (1) compared with the underground water (control), soil pH value with a decrement of 0.21 units in piggery wastewater (PW), and 0.24 units in saline water treatments (SW). Soil electrical conductivity (EC) value significantly increased by 5.8~20.9% in PW and SW treatments, while there was no significant difference in EC in reclaimed water. The highest EC (770 µS/cm) was recorded in SW treatment. (2) No dramatic difference on the concentrations of soil lead (Pb) and cadmium (Cd) in the PW, RW, and SW treatments, compared with the control, but soil organic matter, copper (Cu), and zinc (Zn) concentrations in wastewater treatments were increased by 2.1~43.4%, 24.4~27.0%, and 14.9~21.9%, respectively. (3) There were no significant differences in cucumber yield and quality in RW treatment, while there was a slight decrease by 1.4% in yield in the SW treatment. The highest cucumber yield was observed in PWH treatment, with an increment of 17.5%. In addition, the contents of Vitamin C, soluble sugar, and protein were also improved by PW treatment. In this study, PW treatment showed the strongest ability to promote cucumber yield and quality, thus indicating that piggery wastewater irrigation with 300 kg/ha nitrogen would be the optimal practice in this region. Long-term study is necessary to monitor potential risk of heavy metals on the quality of soil and plant.
The large amount of nitrogen application on the North China Plain has caused a serious negative impact on the sustainable development of regional agriculture and ecological environmental protection. Our aim was to explore the effects of nitrogen fertilization rate and groundwater depth on growth attributes, soil-water and soil-fertilizer contents, and the winter wheat yield. Experiments were carried out in micro-lysimeters at groundwater depths of 60, 90, 120, and 150 cm on the basis of 0, 150, 240, and 300 kg/ha nitrogen fertilization rates in the growth season for winter wheat. Results showed that plant height, leaf area index, soil plant analysis development, and yield without nitrogen application increased significantly with increases in groundwater depth. The optimal groundwater depths for growth attributes and yield were 60–120 cm and tended to be shallower with added nitrogen application. Soil moisture was lowered significantly with groundwater depth, adding a nitrogen application reduced soil moisture, and excessive nitrogen input intensified soil drought. Nitrate-N accumulation at the 120–150 cm depths was significantly higher than that at the 60–90 cm depths, and a 300 kg/ha (traditional nitrogen application rate) treatment was 6.7 times greater than that of 150 kg/ha treatment and increased by 74% more than that of the 240 kg/ha treatment at 60–150 cm depth. Compared with the yield of the 300 kg/ha rate, the yield of the 240 kg/ha rate had no significant difference, but the yield increased by 3.90% and 11.09% at the 120 cm and 150 cm depths. The growth attributes and yield of winter wheat were better, and the soil nitrate-N content was lower, when the nitrogen application rate was 240 kg/ha. Therefore, it can be concluded that nitrogen application can be reduced by 20% on the North China Plain.
Shallow groundwater plays a vital role in physiology morphological attributes, water use, and yield production of winter wheat, but little is known of its interaction with nitrogen (N) application. We aimed to explore the effects of N fertilization rate and shallow groundwater table depth (WTD) on winter wheat growth attributes, yield, and water use. Experiments were carried out in micro-lysimeters at WTD of 0.6, 0.9, 1.2, and 1.5 m with 0, 150, 240, and 300 kg/ha N application levels for the winter wheat (Triticum aestivum L.). The results showed that there was an optimum groundwater table depth (Op-wtd), in which the growth attributes, groundwater consumption (GC), yield, and water use efficiency (WUE) under each N application rate were maximum, and the Op-wtd decreased with the increase in N application. The Op-wtd corresponding to the higher velocity of groundwater consumption (Gv) appeared at the late jointing stage, which was significantly higher than other WTD treatments under the same N fertilization. WTD significantly affected the Gv during the seeding to the regreening stage and maturity stage; the interaction of N application, WTD, and N application was significant from the jointing to the filling stage. The GC, leaf area index (LAI), and yield increased with an increase of N application at 0.6–0.9-m depth—for example, the yield and the WUE of the NF300 treatment with 0.6-m depth were significantly higher than those of the NF150–NF240 treatment at 20.51%, and 14.81%, respectively. At 1.2–1.5-m depth, the N application amount exceeding 150–240 kg/ha was not conducive to wheat growth, groundwater use, grain yield, and WUE. The yield and the WUE of 150-kg/ha treatment were 15.02% and 10.67% higher than those of 240–300-kg/ha treatment at 1.2-m depth significantly. The optimum N application rate corresponding to yield indicated a tendency to decrease with the WTD increase. Considering the winter wheat growth attributes, GC, yield, and WUE, application of 150–240 kg/ha N was recommended in our experiment.
Shallow groundwater plays a vital role in water use and the yield of winter wheat. Nitrogen (N) application significantly affects crop uptake and utilization of water from irrigation, but little is known about groundwater use. More importantly, excessive N application will also bring a series of environmental problems. An experiment was carried out in micro-lysimeters at 0, 150, 240, and 300 kg/ha N fertilization rates based on 0.6 m groundwater depth with relatively strong alkaline soil in the winter wheat growing season. The results showed that increasing the N application rate significantly increased the sensitivity of the daily groundwater evaporation velocity of winter wheat to environmental meteorological factors (soil surface moisture, humidity, atmospheric pressure and atmospheric temperature), and promoted crop water use, crop growth and yield under the 0.6 m groundwater depth. From 150 kg/ha to 300 kg/ha N fertilization, LAI and yield increased by 26.95–82.02%, and evapotranspiration (ET) and groundwater use efficiency (GUE) increased by 11.17–14.38%. However, a high N application rate would sharply induce surface soil drought, leading to a rapid increase in nitrate accumulation in the vadose zone and a significant decrease in partial factor productivity of applied N (PFPN). With the N application of 150–300 kg/ha, the accumulation of nitrate in the vadose zone increased by 8.12 times, and soil moisture in 0–20 cm and PFPN significantly decreased by 19.16–57.53%. N fertilization had a significant effect on water transfer and could promote the consumption and utilization of groundwater at 0.6 m depth. Considering yield, water use, the accumulation of nitrate, and PFPN, the optimal N application was 219.42–289.53 kg/ha at 0.6 m depth.
Rising freshwater scarcities pose a serious threat to agricultural production. Reclaimed water (RW) is increasingly utilized as one of the alternative resources for irrigation in agriculture. Microbial communities play crucial roles in the soil microenvironment and can be used as effective indicators to assess the ecological influence of RW irrigation in soil. However, there is a lack of research on the effects of RW with different irrigation techniques on soil attributes and microbial communities. The present experiment was conducted in China to investigate the effect of two kinds of water qualities (RW and clean water (CW)), two kinds of irrigation methods (full irrigation (FI) and alternate partial root-zone irrigation (APRI)), and two kinds of irrigation techniques (furrow irrigation (FUI) and subsurface drip irrigation (SDI)) on soil chemical properties, heavy metal concentrations, and bacterial community structure. The APRI treatments received 70% of the irrigation water volume of FI. The results revealed that electrical conductivity (EC), nitrate nitrogen (NO3−-N), and heavy metal (Cu, Cd, Pb, Zn) concentrations in soil irrigated with RW were significantly higher in comparison to the soil irrigated with CW. SDI significantly decreased the contents of TN by 4.88%, the EC by 13.78%, and the heavy metal Cd concentration by 13.14% in soils than that irrigated with FUI treatment. APRI significantly decreased the heavy metal Cu concentration in soils by 6.26% compared to FI treatment. Proteobacteria, Chloroflexi, Acidobacteria, and Gemmatimonadetes in soil irrigated with RW were more abundant than that irrigated with CW. The irrigation water quality, soil moisture content, heavy metal content, TN, and EC under various irrigation techniques and methods significantly affected the structure of soil bacterial communities. In conclusion, we highlight that the SDI-APRI treatment can be an efficient irrigation practice for reducing the EC, heavy metal pollution, and the security risks of soil irrigated by RW.
The growing population in conjunction with water scarcity forces us to search for alternative sources of irrigation water and integrate it with irrigation strategies for agricultural expansion to meet sustainable development objectives. For this purpose, a field experiment was conducted over three years (2017, 2018, and 2019) to investigate the effect of water quality (reclaimed water (RW) and freshwater (CW)), irrigation techniques (subsurface drip irrigation (SDI and Furrow irrigation (FUI)), irrigation methods (Full irrigation (FI) and alternate partial root-zone irrigation (APRI (70% ETc)), and their interactions on the fresh fruit yield (FY), irrigation water use efficiency (IWUE), and nitrogen use efficiency (NUE) of tomatoes. As well as evaluate the effects of these experimental factors on soil properties regard to electrical conductivity (EC), pH, and organic matter (OM) of Soil. The experiment was undertaken over three growing spring seasons in China. There were eight treatments in the experiment. For all three years, the yield, IWUE, and NUE values of all treatments under RW were high compared with the corresponding values under CW. The same occurred under SDI compared with FUI. Analysis of variances showed that there was no significant effect (P > 0.05) of water quality, irrigation technique, and irrigation methods on the soil EC, PH, and OM over the three years. In addition, there was no significant effect (P> 0.05) on the interaction between the experimental factors over the three years. In conclusion, the application of RW under SDI can result in saving CW and increasing productivity without any negative effect on the investigated soil properties, as well as, when RW-SDI is used in conjunction with APRI, it can result in increasing IWUE.
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