For large segmental bone defects, porous titanium scaffolds have some advantages, however, they lack electrical activity which hinders their further use. In this study, a barium titanate (BaTiO
3
) piezoelectric ceramic was used to modify the surface of a porous Ti6Al4V scaffold (pTi), which was characterized by scanning electron microscopy, energy dispersive spectroscopy, X-ray photoelectron spectroscopy, and roughness and water contact angle analyses. Low intensity pulsed ultrasound (LIPUS) was applied in vitro and in vivo study. The activity of bone marrow mesenchymal stem cells, including adhesion, proliferation, and gene expression, was significantly superior in the BaTiO
3
/pTi, pTi + LIPUS, and BaTiO
3
/pTi + LIPUS groups than in the pTi group. The activity was also higher in the BaTiO
3
/pTi + LIPUS group than in the BaTiO
3
/pTi and pTi + LIPUS groups. Additionally, micro-computed tomography, the mineral apposition rate, histomorphology, and the peak pull-out load showed that these scaffold conditions significantly enhanced osteogenesis and osseointegration 6 and 12 weeks after implantation in large segmental bone defects in the radius of rabbits compared with those resulting from the pTi condition. Consequently, the improved osteogenesis and osseointegration make the BaTiO
3
/pTi + LIPUS a promising method to promote bone regeneration in large segmental bone defects for clinical application.
ZSM-5 zeolite nanoboxes with accessible mesomicro-pore architecture and strong acid sites are important in relevant heterogeneous catalysis suffering from mass transfer limitations and weak acidities.R ational design of parent zeolites with concentrated and non-protective coordination of Al species can facilitate post-synthetic treatment to produce mesoporous ZSM-5 nanoboxes.I nt his work, as imple and effective method was developed to convert parent MFI zeolites with tetrahedral extra-framework Al into Al-enriched mesoporous ZSM-5 nanoboxes with low silicon-to-aluminium ratios of % 16. The parent MFI zeolite was prepared by rapid ageing of the zeolite sol gel synthesis mixture.The accessibility to the meso-micro-porous intra-crystalline network was probed systematically by comparative pulsed field gradient nuclear magnetic resonance diffusion measurements,w hich, together with the strong acidity of nanoboxes,provided superb catalytic activity and longevity in hydrocarbon cracking for propylene production.
Plasma-catalysis
systems are complex and require further understanding
to advance the technology. Herein, CO poisoning in CO2 hydrogenation
over supported ruthenium (Ru) catalysts in a nonthermal plasma (NTP)-catalysis
system was investigated by a combined kinetic and diffuse reflectance
infrared Fourier transform spectroscopy–mass spectrometry (DRIFTS–MS)
study and compared with the thermal catalytic system. The relevant
findings suggest the coexistence of the Langmuir–Hinshelwood
and Eley–Rideal mechanisms in the NTP-catalysis. Importantly,
comparative study of CO poisoning of the Ru catalyst was performed
under the thermal and NTP conditions, showing the advantage of the
hybrid NTP-catalysis system over the thermal counterpart to mitigate
CO poisoning of the catalyst. Specifically, compared with the CO poisoning
in thermal catalysis due to strong CO adsorption and associated metal
sintering, in situ DRIFTS–MS analysis revealed
that the collisions of reactive plasma-derived species in NTP-catalysis
could remove the strongly adsorbed carbon species to recover the active
sites for CO2 activation. Thus, the NTP-catalysis was capable
of preventing CO poisoning of the Ru catalyst in CO2 hydrogenation.
Additionally, under the NTP conditions, the NTP-enabled water-gas
shift reaction of CO with H2O (which was produced by CO/CO2 hydrogenation) shifted the equilibrium of CO2 hydrogenation
toward CH4 production.
A systematic study of Ni supported on metal-organic frameworks (MOFs) catalyst (i.e., 15Ni/UiO-66) for catalytic CO 2 hydrogenation under nonthermal plasma (NTP) conditions was presented. The catalyst outperformed other catalysts based on conventional supports such as ZrO 2 , representing highest CO 2 conversion and CH 4 selectivity at about 85 and 99%, respectively. We found that the turnover frequency of the NTP catalysis system (1.8 ± 0.02 s −1) has a nearly twofold improvement compared with the thermal catalysis (1.0 ± 0.06 s −1). After 20 hr test, XPS and HRTEM characterizations confirmed the stability of the 15Ni/UiO-66 catalyst in the NTPactivated catalysis. The activation barrier for the NTP-activated catalysis was calculated as~32 kJ mol −1 , being lower than the activation energy of the thermal catalysis (~70 kJ mol −1). In situ DRIFTS characterization confirmed the formation of multiple carbonates and formates on catalyst surface activated by NTP, surpassing the control catalysts (e.g., 15Ni/α-Al 2 O 3 and 15Ni/ZrO 2).
Bone
defect repair at load-bearing sites is a challenging clinical
problem for orthopedists. Defect reconstruction with implants is the
most common treatment; however, it requires the implant to have good
mechanical properties and the capacity to promote bone formation.
In recent years, the piezoelectric effect, in which electrical activity
can be generated due to mechanical deformation, of native bone, which
promotes bone formation, has been increasingly valued. Therefore,
implants with piezoelectric effects have also attracted great attention
from orthopedists. In this study, we developed a bioactive composite
scaffold consisting of BaTiO3, a piezoelectric ceramic
material, coated on porous Ti6Al4V. This composite scaffold showed
not only appropriate mechanical properties, sufficient bone and blood
vessel ingrowth space, and a suitable material surface topography
but also a reconstructed electromagnetic microenvironment. The osteoconductive
and osteoinductive properties of the scaffold were reflected by the
proliferation, migration, and osteogenic differentiation of mesenchymal
stem cells. The ability of the scaffold to support vascularization
was reflected by the proliferation and migration of human umbilical
vein endothelial cells and their secretion of VEGF and PDGF-BB. A
well-established sheep spinal fusion model was used to evaluate bony
fusion in vivo. Sheep underwent implantation with different scaffolds,
and X-ray, micro-computed tomography, van Gieson staining, and elemental
energy-dispersive spectroscopy were used to analyze bone formation.
Isolated cervical angiography and visualization analysis were used
to assess angiogenesis at 4 and 8 months after transplantation. The
results of cellular and animal studies showed that the piezoelectric
effect could significantly reinforce osteogenesis and angiogenesis.
Furthermore, we also discuss the molecular mechanism by which the
piezoelectric effect promotes osteogenic differentiation and vascularization.
In summary, Ti6Al4V scaffold coated with BaTiO3 is a promising
composite biomaterial for repairing bone defects, especially at load-bearing
sites, that may have great clinical translation potential.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.