Osteosarcoma is the most common cause of cancer-associated mortality and the prognosis is yet to be fully elucidated due to the paucity of effective therapeutic targets that significantly influence the quality of life and mean survival rates of patients with osteosarcoma. Studies have showed that tripartite motif-containing (TRIM)-14 is a member of the TRIM protein family that has a vital role in tumor progression and metastasis and promotes angiogenesis, invasion and apoptotic resistance of bone cancer. In this study, a chimeric antibody targeting TRIM-14 (Chanti-TRIM) was constructed and the molecular mechanism of target therapy for TRIM-14 was investigated in osteosarcoma cells and xenograft mice. The growth, migration and invasion properties of U-2OS cells were analyzed following incubation with 10–160 mg/ml Chanti-TRIM. Apoptosis of U-2OS cells was detected after Chanti-TRIM treatment. Matrix metalloproteinase (MMP)-9-mediated nuclear factor-κB (NF-κB) signal pathway was analyzed in U-2OS cells treated with Chanti-TRIM. The inhibitory efficacy of Chanti-TRIM was studied in U-2OS-bearing xenograft mice. Our results demonstrated that neutralizing TRIM-14 expression markedly inhibited the growth, migration and invasion of osteosarcoma cells, in vitro and in vivo. We found that TRIM-14 depletion decreased cell viability and induced cells apoptosis in vitro. In addition, we identified Chanti-TRIM inhibited growth and promoted apoptosis induced by cisplatin through MMP-9-mediated NF-κB signal pathway. Furthermore, we observed that Chanti-TRIM treatment inhibited osteosarcoma growth in vivo. Histological analysis indicated that apoptotic bodies were increased and NF-κB nuclear translocation factors, including Ikkβ, p65 and IkBα, were decreased in tumors treated by Chanti-TRIM. In conclusion, these results showed that Chanti-TRIM markedly inhibited the progression of osteosarcoma, suggesting Chanti-TRIM may be a potential anti-cancer agent that functions via the activation of the NF-κB pathway for osteosarcoma.
There are usually two forms of bone repair materials, block and granular, for common clinical use. This paper describes a novel injectable material, nano-HA/collagen/alginate (nHAC/Alg) composite biomaterial, including its preparation and evaluations in vitro. Based on the idea of bionics and the study of collagen/calcium phosphate salt composite materials, the injectable bone repair material was developed. Then, human bone marrow stem cells (hBMSCs) were cultured on the nHAC/Alg material. The cell attachment, proliferation, and differentiation were evaluated with inverted microscope, scanning electron microscope, laser scanning confocal microscope, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) analysis, and alkaline phosphatase (ALP) test. The results showed that nHAC/Alg not only had no negative effect on cellular functions but also promotes cell proliferation and differentiation into osteogenic cells, which suggests that the nanoscaled injectable bone repair material has good clinical application prospects for bone repair.
In this work, BiOI, BiPO 4 , BiPO 4 /BiOI (M) and MWCNT/BiPO 4 /BiOI(MWCNT/M) heterostructure were synthesized through hydrothermal methods and served the function of fabricating photoelectrochemical (PEC) sensor for detection of Salicylic acid (SA). MWCNT covered with BiPO 4 /BiOI were observed with the aid of scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Under visible light excitation, the low detection limit of 0.55 μM and working range of 1-320 μM and 480-3840 μM were obtained at the MWCNT/M/ITO. Moreover, it has excellent reproducibility and long-term stability. Meanwhile, results suggest that MWCNT/M developed herein offers a promising platform for the ultrasensitive detection of SA.
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