RNA-Puzzles is a collective endeavor dedicated to the advancement and improvement of RNA 3D structure prediction. With agreement from crystallographers, the RNA structures are predicted by various groups before the publication of the crystal structures. We now report the prediction of 3D structures for six RNA sequences: four nucleolytic ribozymes and two riboswitches. Systematic protocols for comparing models and crystal structures are described and analyzed. In these six puzzles, we discuss (i) the comparison between the automated web servers and human experts; (ii) the prediction of coaxial stacking; (iii) the prediction of structural details and ligand binding; (iv) the development of novel prediction methods; and (v) the potential improvements to be made. We show that correct prediction of coaxial stacking and tertiary contacts is essential for the prediction of RNA architecture, while ligand binding modes can only be predicted with low resolution and simultaneous prediction of RNA structure with accurate ligand binding still remains out of reach. All the predicted models are available for the future development of force field parameters and the improvement of comparison and assessment tools.

This study investigates the relationships among website quality, website brand, perceived trust, perceived value, and purchase intention in the hotel industry. The study sample was a target chain hotel in Taiwan (i.e., Sheraton). A statistical analysis of the collected questionnaires was computed from a web-based survey. Using the structural equation modeling (SEM) technique, the results reveal that perceived trust is positively influenced by website quality, and purchase intention is positively influenced by perceived trust. Therefore, purchase intention is indirectly influenced by website quality through the mediator of perceived trust. Furthermore, the relationship between website quality and perceived trust is stronger for customers who perceive better website brand while the relationship between perceived trust and purchase intention is found to be stronger for customers who perceive high service value. According to the findings, the implications and future research directions are provided.

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