Featured Application: This biosensor was used for the detection of dopamine without interference from high concentrations (0.5 mM) of ascorbic acid. In addition, the electrode developed in this study presented a great sensitivity (22 nM) and a broad linear range compared with existing electrochemical sensors in the detection of dopamine. Moreover, the analysis of dopamine in physiological samples was examined.Abstract: Tyrosinase, chitosan, and reduced graphene oxide (rGO) are sequentially used to modify a screen-printed carbon electrode (SPCE) for the detection of dopamine (DA), without interference from uric acid (UA) or ascorbic acid (AA). The use of tyrosinase significantly improves the detection's specificity. Cyclic voltammetry (CV) measurements demonstrate the high sensitivity and selectivity of the proposed electrochemical sensors, with detection limits of 22 nM and broad linear ranges of 0.4-8 µM and 40-500 µM. The fabricated tyrosinase/chitosan/rGO/SPCE electrodes achieve satisfactory results when applied to human urine samples, thereby demonstrating their feasibility for analyzing DA in physiological samples.
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