ABSTRAK KONVERSI KORAL LAUT MENJADI HIDROKSIAPATIT DENGAN METODE SONIKASI.Sintesis coralline hydroxyapatite (CHAp) dari koral laut yang umumnya menggunakan metode basah yaitu hidrotermal yang memerlukan suhu dan tekanan tinggi. Tujuan dari penelitian ini adalah mengkonversi koral laut Goniopora sp. menjadi CHAp dengan metode sonikasi pada suhu rendah. Goniopora sp. dikalsinasi pada suhu 900°C selama 3 jam, kemudian direaksikan dengan diammonium hidrogen fosfat [(NH4)2HPO4] dan mono kalium fosfat (KH2PO4) dengan waktu sonikasi 6 jam sampai 24 jam pada suhu 60°C. Hasil penelitian menunjukkan bahwa telah terjadi perubahan fase dari koral menjadi CHAp yang dapat dilihat dari identifikasi pola difraksi sinar-X khas dari hidroksiapatit dengan derajat kristalinitas sebesar 66%
Objective The aim of this study was to evaluate the effect of gamma-ray irradiation on the physical, mechanical, and morphological characteristics of the polyvinyl alcohol (PVA)-collagen-chitosan membranes as a guided tissue regeneration membrane material.
Material and Method The membrane was fabricated by mixing PVA, collagen, and chitosan using the film casting method. PVA-collagen-chitosan membranes were irradiated with various radiation dose (0, 15, and 25 kGy). Furthermore, it is characterized using Fourier-transform infrared (FTIR) for functional group identification, morphological test was performed using scanning electron microscopy (SEM), and mechanical properties (i.e., tensile strength and elongation) were evaluated using universal testing machine and swelling studies.
Statistical Analysis Statistical analysis was performed based on analysis of variance and post hoc with p-value < 0.05.
Result The FTIR spectrum shows various peaks of functional groups from the PVA-collagen-chitosan membrane. The result of the statistical analysis show changes in tensile strength (p = 0.0004) and membrane elongation (p = 0.000451) at different radiation doses of 0, 15, and 25 kGy. The membrane absorption obtains p-value of 0.0193, while the SEM results show that the PVA-collagen-chitosan membrane homogeneously mixed.
Conclusion There is an effect of gamma-ray irradiation on tensile strength, elongation, and water absorption of the membranes. Increasing the radiation dose increases the value of tensile strength, while elongation and absorption of the membrane decrease. The PVA-collagen-chitosan membrane has the potential to develop as an alternative membrane for guided tissue regeneration.
ι-Carrageenan is a biodegradable and biocompatible biomaterial which potentially stabilizes colloidal silver nanoparticles (AgNPs). The present study explored gamma radiosynthesis of AgNPs at varied concentration of ι-carrageenan solutions. The reaction system contained 1.0 mM silver precursor from silver nitrate salt. Gamma irradiation was conducted at doses up to 20 kGy under simple condition, i.e., atmospheric gases and without addition of hydroxyl radical scavenger. The behavior of AgNPs in suspension was characterized based on their surface plasmon resonance (SPR) absorption spectra which were measured using UV-vis spectrophotometer. The results show that colloidal AgNPs were successfully radiosynthesized due to dual stabilizing/reducing activity of ι-carrageenan. The degradation product of ι-carrageenan shows antioxidant activities, which increase the reducing condition of the reaction system. TEM micrograph reveals that the nanoparticles are spheroid in shape and monodisperse with an average particle size of below 10 nm. The SPR spectra indicate that the highest AgNPs concentration is found for irradiation at a dose of 10 kGy and ι-carrageenan concentration of 1.0 % (w/v). However, instability of AgNPs occurred a day after radiosynthesis due to oxidative dissolution and agglomeration. Further works on pH adjustment and optimization on irradiation dose and ι-carrageenan concentration are critical to improve the stability of colloidal AgNPs.
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