The Effect of Gamma-Ray Irradiation on the Physical, Mechanical, and Morphological Characteristics of PVA-Collagen-Chitosan as a Guided Tissue Regeneration (GTR) Membrane Material

Abstract

Objective The aim of this study was to evaluate the effect of gamma-ray irradiation on the physical, mechanical, and morphological characteristics of the polyvinyl alcohol (PVA)-collagen-chitosan membranes as a guided tissue regeneration membrane material. Material and Method The membrane was fabricated by mixing PVA, collagen, and chitosan using the film casting method. PVA-collagen-chitosan membranes were irradiated with various radiation dose (0, 15, and 25 kGy). Furthermore, it is characterized using Fourier-transform infrared (FTIR) for functional group identification, morphological test was performed using scanning electron microscopy (SEM), and mechanical properties (i.e., tensile strength and elongation) were evaluated using universal testing machine and swelling studies. Statistical Analysis Statistical analysis was performed based on analysis of variance and post hoc with p-value < 0.05. Result The FTIR spectrum shows various peaks of functional groups from the PVA-collagen-chitosan membrane. The result of the statistical analysis show changes in tensile strength (p = 0.0004) and membrane elongation (p = 0.000451) at different radiation doses of 0, 15, and 25 kGy. The membrane absorption obtains p-value of 0.0193, while the SEM results show that the PVA-collagen-chitosan membrane homogeneously mixed. Conclusion There is an effect of gamma-ray irradiation on tensile strength, elongation, and water absorption of the membranes. Increasing the radiation dose increases the value of tensile strength, while elongation and absorption of the membrane decrease. The PVA-collagen-chitosan membrane has the potential to develop as an alternative membrane for guided tissue regeneration.

Synthesis and Structural Characterization of Four Different Concentrations of Ant Nest (Myrmecodia pendens) Collagen Membranes with Potential for Medical Applications

“…The collagen-based gel from tilapia fish scales exhibited anti-inflammatory activity in the healing process of incision wounds. [16][17][18] The use of collagen as barrier membranes in GBR (Guided Bone Regeneration) has been widely practiced. Membranes prepared using collagen-chitosan from white snapper scales affected wound healing, as evidenced by increased fibroblasts and new blood vessel formation.…”

Mechanical, degradation rate, and antibacterial properties of a collagen-chitosan/PVA composite nanofiber

“…Another advantage is that PVA can form excellent fibers through electrospinning [16]. Previous studies regarding Collagen-chitosan/PVA were still in the form of hydrogels and thin films [17,18]. However, the research on Collagen-chitosan/PVA composites in nanofiber structures has not been widely reported.…”

Determination of the Sterilization Dose of Gamma-Ray Irradiation for Polyvinyl Alcohol-Collagen-Chitosan Composite Membrane as a Material for Periodontal Regenerative Surgery

“…1 2 3 4 Membrane barrier usage was commenced when the GTR concept was introduced, with its main function to inhibit epithelial cells migration by placing the membrane between the flap and root surface, thereby preventing connective tissue contact and isolating the cells to restore the periodontal ligament, cementum, and connective tissue. 5 6…”

“…[1][2][3][4] Membrane barrier usage was commenced when the GTR concept was introduced, with its main function to inhibit epithelial cells migration by placing the membrane between the flap and root surface, thereby preventing connective tissue contact and isolating the cells to restore the periodontal ligament, cementum, and connective tissue. 5,6 In general, barrier membranes are divided into two, namely nonresorbable and resorbable. The use of resorbable membranes in recent years has increased as this reduces the need for a second surgical procedure.…”