One of the producers of honey bees in North Sulawesi comes from the Manembo Forest, South Minahasa. Today many ordinary people do not know the quality of good honey. This study aims to study the characteristics of bee honey from the South Minahasa Manembo Forest based on SNI. The research was conducted in April-July 2022. The research method used for testing the quality of honey was using qualitative research methods. The data obtained were then analyzed using descriptive data analysis. Determination of total flavonoid levels using quercetin as a comparison. The absorbance value was measured by UV-Vis spectro-photometer. The absorbance value is then entered into the linear regression equation. The results showed that the characteristics of Manembo forest honey, South Minahasa Regency based on SNI 2018 parameters met the standards and quality with the percentage of reducing sugar 68.53%, sucrose content 3.07%, insoluble solids 0.14%, diastase enzyme DN=4 and 0.03% ash content while those that do not meet the standard are water content with a percentage of 25%. The results of the analysis of the total flavonoid content showed a value of 1.6 mgQE/g. For further research, it is recommended to make an antidiabetic or anti-bacterial analysis in forest honey.
Thermophilic bacteria live at temperatures above 450 C. Many investigations focused on their potential as sources of highly active enzymes ‘termostable enzyme’ and other products such as antibiotics and compatible solutes. Lake Linow is an active volcanic lake located in Tomohon City, North Sulawesi, Indonesia. Lake Linow becomes the habitat of thermophilic bacteria. A study has been conducted to obtain isolates of thermophilic bacteria and to identifikasi berdasarkan gen 16 s RNA. Bacterial DNA extraction procedure using the Presto TM Mini gDNA Bacteria Kit Geneaid protocol, with modifications. Amplification of 16s RNA gene using PCR method. Visualization of 16 s RNA amplicon genes with automatic electrophoresis capiler Qiaxel, Qiagen. Sequencing was carried out using Singapore's First BASE Sequencing service. The results showed that IL2 isolates and IL3 isolates could live up to 700C. Alignment analysis results using NCAST BLBI IL2 isolates showed 99% similarity with Bacillus thuringiensis strain H2682 (accession number CP009720.1). While isolate of IL3 thermophilic bacteria showed 94% similarity with Bacillus licheniformis strain 14DA11 (accession number CP023168.1). The results of phylogeny reconstruction with neighbor joining method, gene sequence 16S rRNA isolate IL2 showed the closest relation with Bacillus thuringiensis strain HD1011 (accession number CP009335.1). While IL3 isolate showed the closest relation with Bacillus licheniformis strain 14DA11 (accession number CP023168.1).
Leilem (Clerodendrum minhassae Teism. & Binn.) is a plant endemic to Sulawesi. Leilem leaves are used as a typical food vegetable for the Minahasa tribe. However, leilem leaves were used as a medicinal plant for intestinal worms, abdominal pain and chest pain. There has been no research report on the use of leilem leaves as a source of antibacterial bioactivity. This study aims to obtain the best concentration of antibacterial Leilem leaf extract. Leilem leaves are obtained from North Minahasa. Extraction by maceration method using various solvents with a level of polarity. The test concentrations of the extract were 100 mg/L, 200 mg/L, 400 mg/L and 800 mg/L. As a positive control, clindamycin 400 mg/L was used. The results showed that the percentage of solvent yield was ethanol, which was 1.23% at 1:4 (w/v) maceration. The average diameter of bacterial growth is 12.6 mm. The results of the one way ANOVA analysis showed significant differences between test concentrations (p>0.5). Tukey's test showed that the three extracts significantly inhibited the growth of gram-positive bacteria Staphylococcus aureus strain ATCC 25923. The n-hexane extract showed the best antibacterial activity, followed by ethanol extract and ethyl acetate extract. Leilem leaves are potential to be developed as a source of antibacterial bioactive.
The Crested black macaque (Macaca nigra) is an endemic species to the Sulawesi Island, limited distribution, and Critically Endangered on the IUCN red list. The population density in the Tangkoko Conservation Forest Management Unit (CFMU) has decreased significantly due to habitat destruction and hunting. Existing data are based on the study in the northern part of Tangkoko. Currently, data from the southern is urgently needed so that population data covers the entire area, including to identify the habitat quality. The aims of this study was to (1) analyze the population density level of Crested black macaque at the Tangkoko CFMU, (2) identify the habitat characterize of Crested black macaque at the Tangkoko CFMU, (3) analyze the correlation between Crested black macaque density and habitat quality at the Tangkoko CFMU. The study was conducted from January to March 2020 in the sothern of Tangkoko CFMU, North Sulawesi. The data collection on the density of Crested black macaque is done by animals directly inventorying through surveys in the field. Observations are carried out by walking at speeds of 1 - 1.5 km / h on each track and stopping at every 100 m to observe the surroundings. Data recorded is the number of individuals found using the line transect method. Vegetation data collection method used is habitat quality data collection carried out by making sample plots measuring 25 m x 25 m for tree level observations in lines with each distance between plots is 250 m. Data recorded for dominance of vegetation are species names, number of individuals, and diameter at breast height of trees. The results showed that (1) the population density level of Crested black macaque was 15 individuals/km2. The highest amount of density is found on the D4 was 72 individu/km2, the lowest on D1, D5, D6, and D7 with no macaques; (2) vegetation analyzed at 45 plots were important value index with the highest value of 86,95 is Ficus variegata on line D7 for tree level, and Theobroma cacao for pole level with highest value of 120,80 on line D4; (3) the correlation between density of Crested black macaque and habitat quality that shows a positive value based on the Pearson correlation test. Further research is needed to determine the relationship between population density, habitat disturbance, and hunting pressure.
Growth hormone regulates reproduction and growth in mammals. A study was conducted to obtain the characteristics of the GH gene, in local pigs in North Sulawesi. Pig samples were obtained from traditional farmers, from four districts in North Sulawesi. DNA extraction and purification, using pig pituitary tissue. Amplification of GH gene, performed by PCR method. Visualization of CO1 gene amplikon, performed by electrophoresis technique. Sequencing, conducted through the First BASE Singapore sequencing service. The results show that there is a variation of local pigs CO1 gene in North Sulawesi. Variations are also found in the amino acid sequence encoded by the GH gene. Knowledge of the characteristics of local pig gh gene, the basics of selection of local pigs superior to North Sulawesi. Key words : growth hormone gen, local pigs, Sulawesi Utara Abstrak Gen growth hormone meregulasi reproduksi dan pertumbuhan pada mamalia. Telah dilaksanakan penelitian yang bertujuan untuk mendapatkan karakteristik gen GH pada babi lokal di Sulawesi Utara. Sampel babi diperoleh dari peternak tradisonal dari empat kabupaten di Sulawesi Utara. Ekstraksi dan purifikasi DNA menggunakan jaringan hipofisis babi. Amplifikasi gen GH menggunakan metode PCR. Visualisasi amplikon gen CO1 dilakukan dengan teknik elektroforesis. Sekuensing dilakukan melalui jasa layanan sekuensing First BASE Singapura. Hasil penelitian menunjukkan bahwa terdapat variasi gen CO1 babi lokal di Sulawesi Utara. Variasi juga ditemukan pada urutan asam amino yang dikode oleh gen GH. Diketahuinya karakteristik gen GH babi lokal, menjadi dasar seleksi babi lokal unggul Sulawesi Utara.
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