Philogenic Relationship of Wild Pigs and Local Pig from North Sulawesi Based on the Growth Hormone Gene (GH Gene)

Revolson, Alexius Mege; Mokosuli, Yermia Semuel; Debby, Jacqueline Jochebed Rayer; Adil, Ellen Hetie; Rompas, Christny; Manampiring, Nony; Montolalu, Merry

doi:10.4028/www.scientific.net/msf.967.71

Cited by 3 publications

(1 citation statement)

References 16 publications

(9 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, the addition of the DNA analysis process to the media content is carried out by practicing the process directly at the UNIMA Biomolecular and Biomolecular Laboratory. The DNA analysis process includes 4 main stages, namely DNA extraction and purification, DNA amplification, electrophoresis and sequencing (Revelson et al, 2019, Suddin, Mokosuli, Marcelina, Orbanus, & Ardi, 2019. The 16S rRNA and CO1 genes have been proven to identify giant freshwater prawn Macrobrachium rosenbergii populations in East Africa (Kuguru, Groeneveld, Singh, & Mchomvu, 2019).…”

Section: Discussionmentioning

confidence: 99%

Development of Audio Visual Learning Mediaof Biology on the Concept of DNA, Based on the Results of Molecular Identification of Payangka Fish from Lake Tondano

2019

EUROPEAN J HEALT BIO ED

View full text Add to dashboard Cite

Payangka fish [Ophieleotrisaporos (Bleeker)] is one of the endemic species in Lake Tondano, Minahasa Regency, North Sulawesi. Empirically, Payangka fish is widely known by the Minahasa community. Identification of fish molecule has not been reported. This research begins with the molecular identification of Payangka fish and is developed in the form of audiovisual media for learning genetic material in high school. The topic of molecular genetic studies has one of biology learning that is considered complicated in high school. The characteristics of DNA material were abstract and were a combination of biological, physical and chemical studies. Practical activities of DNA analysis using molecular identification methods in high schools are difficult because they require sophisticated tools. Nevertheless, the concept of DNA as a key material in molecular biology is becoming a standard in the international biology curriculum. This research provides a solution by developing a molecular identification practicum carried out at the Unima FMIPA Biology laboratory and packaged in audiovisual media. Another advantage is the object of molecular identification, namely Payangka fish is an endemic species of Lake Tondano. This type of research was development research proposed by Tiangarajan, the 4D model. As the name implies, there were 4 stages in this research, namely Define, Design, Development, and Disseminate. To test the validity of this learning media, a trial is carried out. The test subjects consisted of two material experts, two instructional media experts, one subject teacher, and ten students for field trials. Data on the feasibility of this learning media product was collected by rubric and team evaluation. The collected data will then be analyzed with qualitative descriptive techniques and descriptive statistics. The results of this study were to indicate the level of eligibility or product validity is in very good criteria with a percentage of 92.87%.

show abstract

Section: Discussionmentioning

confidence: 99%

Development of Audio Visual Learning Mediaof Biology on the Concept of DNA, Based on the Results of Molecular Identification of Payangka Fish from Lake Tondano

2019

EUROPEAN J HEALT BIO ED

View full text Add to dashboard Cite

show abstract

Population connectivity and genetic diversity population connectivity of Rabbitfish (Siganus canaliculatus) among Bangladesh, China and Indonesia

Putri¹,

Maharani²,

Farhan³

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

View full text Add to dashboard Cite

Population genetics studies genetic variation involving gene and allele frequencies in spatial and temporal space. Genetic information can be used in conservation management through identification of phylogenetic tree reconstructions, genetic distance, genetic structure, and haplotype connectivity to maintain population existence. The samples used were primary genetic data of Siganus canaliculatus taken from Indonesian waters and secondary data accessed from the BOLD SYSTEM database from several locations, namely Indonesia, China, and Bangladesh. This research was conducted to determine the kinship of Siganus canaliculatus in Indonesia, China, and Bangladesh through genetic analysis. The results of genetic distance analysis showed that the baronang fish samples from China and Bangladesh had a close relationship with a value of 0.0534 while samples from Indonesia and Bangladesh had the farthest relationship with a value of 0.8212. Reconstruction of the phylogeny tree at maximum likelihood can show convergence within the same clade. The results of the reconstruction of the phylogeny tree showed that the species from Ambon, Bali, and Lombok came from the same clade while samples from Bangladesh had different clades with the furthest distance. The results of structural analysis and genetic diversity showed that samples from Bangladesh, China, and Indonesia had different DNA characteristics. This is correlated with the form of network integration in haplotype connectivity analysis.

show abstract

Isolation, Phenotypic Identification and Antibiotic Resistance Profile of Bacterial Isolates from Intestinal Fluids of Local Minahasa Pigs, North Sulawesi, Indonesia

Mege¹,

Mokosuli²,

Manampiring³

et al. 2022

J. Pure Appl. Microbiol.

View full text Add to dashboard Cite

The Minahasa local pig is unique because it is the oldest local pig that has spread to the Philippine islands. Minahasa local pigs have endemic characteristics because they are in the Wallacea zone. Research has been carried out to isolate bacteria from pig intestinal fluid and obtain an antibiotic response profile from pure bacterial isolates. Pig samples were obtained from two locations in North Sulawesi, namely in North Minahasa and North Minahasa. Intestinal fluids are taken immediately when the pig is slaughtered. The liquid was preserved in a sterile container and inoculated directly on the nutrient agar medium by the dilution method. The bacterial isolates obtained were pure cultured and then used for automatic phenotypic identification using Vitek 2 Compact. The results showed that pure culture isolates were obtained from intestinal fluids of local pigs in Minahasa, North Sulawesi, indicating that two isolates (S1 and S2) were Escherichia coli and S3 was Enterobacter aerogenes. Isolate S1 showed resistance to Ampicillin, while isolate S2 showed resistance to Tetracycline, furthermore isolate S3 showed resistance to Tetracycline, Furanes, and Trimethoprim/Sulfonamide. The results confirm that further research is needed to isolate and test the antibiotic resistance of bacteria from pig intestines in several locations and various stages of common local pigs.

show abstract

Philogenic Relationship of Wild Pigs and Local Pig from North Sulawesi Based on the Growth Hormone Gene (GH Gene)

Cited by 3 publications

References 16 publications

Development of Audio Visual Learning Mediaof Biology on the Concept of DNA, Based on the Results of Molecular Identification of Payangka Fish from Lake Tondano

Development of Audio Visual Learning Mediaof Biology on the Concept of DNA, Based on the Results of Molecular Identification of Payangka Fish from Lake Tondano

Population connectivity and genetic diversity population connectivity of Rabbitfish (Siganus canaliculatus) among Bangladesh, China and Indonesia

Isolation, Phenotypic Identification and Antibiotic Resistance Profile of Bacterial Isolates from Intestinal Fluids of Local Minahasa Pigs, North Sulawesi, Indonesia

Contact Info

Product

Resources

About