PurposeRespiratory syncytial virus (RSV) can cause serious respiratory illnesses such as pneumonia, asthma, and bronchiolitis in infants and elderly or immunocompromised individuals. An RSV vaccine has yet to be developed; only prophylactic anti-RSV antibody is commercially available. So, we investigated whether our vaccine candidate is able to induce type 1 CD4+ T helper (Th1), CD8+ T-cell responses, and protective immunity without vaccine-enhanced disease (VED) against RSV.Materials and MethodsWe used RSV G protein fragment (Gcf A) with recombinant baculovirus capable of expressing the RSV M2 protein (Bac M2) as a vaccine candidate, and injected this vaccine (Gcf A/Bac M2) intramuscularly, and challenged with RSV intranasally into mice. Enzyme-linked immunosorbent assay, flow cytometry, plaque assay, and weight measurement were performed to confirm humoral immunity, cellular immunity, and protective immunity.ResultsThe Gcf A/Bac M2 formulation induced a stronger IgG response to Gcf A than Gcf A inoculation alone, and the ratio of IgG1/IgG2a indicated that the responses shifted predominantly to Th1. In addition, both RSV G-specific Th1 responses and RSV M2-specific CD8+ T-cell responses were induced, and G protein-associated eosinophilic infiltration was suppressed compared to the control group. Moreover, the Gcf A/Bac M2 group showed effective protection after an RSV challenge.ConclusionBac M2 could serve as a vaccine with intrinsic adjuvant activity, and the Gcf A/Bac M2 shows promise as a vaccine candidate for inducing protective immunity without inciting VED.
A soybean cultivar, "Seonyu2ho" for double cropping using soy-paste and tofu was developed in 2011 using the pedigree method by crossing "Milyang235ho" and "Jungmo3004ho." A promising line, YS2441-B-2S-11-3, was selected and named as "Milyang334." It demonstrated prominent early maturing, shattering resistance, and high yield in regional yield trials (RYTs) from 2017 to 2019, and was released by the name "Seonyu2ho." The cultivar showed a determinate growth habit, white flowers, grey pubescence, yellow seed coat, yellow hilum, flat-spherical seed shape, and large seed size (27.9 g per 100 seeds). "Seonyu2ho" matured on October 5 (112-growing day cycle) in RYT and was suitable for double cropping with winter crops, such as wheat, barley, and onion. It was resistant to bacterial pustule and soybean mosaic virus and tolerant to lodging in fields. Particularly, "Seonyu2ho" showed a high yield (3.34 ton/ha) and shattering resistance, even if the previously developed early matured cultivars exhibited low yield and easy shattering. Overall, "Seonyu2ho" can contribute to stable soybean production in double cropping.
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