Little attention has been paid to the problem of the combined toxicity of accumulated antibiotics on humans from food and clinical treatments. Therefore, we used human hepatocytes to study the joint toxicity of four common antibiotics. The cytotoxicity of enrofloxacin (ENR), combined with ciprofloxacin (CFX), florfenicol (FFC), or sulfadimidine (SMD) on THLE-2 cells was determined by CCK-8 assays; then their joint toxicity was evaluated using CalcuSyn 2.0. Dose–effect curves and median-effect plots established on large amounts of data and CI values were calculated to judge the nature of the combination’s interaction. ED50, ED75, and ED90 were predicted to elucidate the changing trend of the concentration on the toxicity of each drug pair. The ENR-CFX and ENR-FFC pairs exhibited synergistic toxicity only at special concentration rates, while ENR and SMD synergistically induced cytotoxicity at almost all the concentration rates studied. The mixed ratio was a significant factor for synergistic toxicity and should be evaluated in all combined effect studies. These results suggested that the combined toxicity of these four drugs should be taken into account in their risk assessment.
Objective:
Controlling coccidiosis disease in poultry is significant for healthy breeding, but antibiotic residues can be a great threat to food safety, the ecological environment, and human health. A special anticoccidial drug known as Hainanmycin has been widely used in Asian countries for many years, while a few studies focus on its detection. In this study, we established a hydrophilic-lipophilic balance (HLB) purification and ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method to determine the hainanmycin in chicken liver.
Methods:
It was extracted from the sample with 0.1% formic acid-methanol (10+90, v/v) and then it was purified on a hydrophilic-lipophilic balance cartridge. Chromatography separation was performed on an RP-C18 column, and detection of the hainanmycin was done by positive electrospray ionization (ESI+) under the multiple reaction monitoring (MRM) modes.
objective:
Controlling coccidiosis disease in poultry is significant for healthy breeding, but antibiotics abuse could carry the risk of these residues. A special anticoccidial drug, hainanmycin, has been widely used in Asia countries for many years while little attention has been paid to it. We established a hydrophilic-lipophilic balance (HLB) purification and ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method to determine hainanmycin in chicken liver.
Results:
The quantification ion pair was m/z 907.5 and m/z 846.4. The calibration curve showed excellent linearity in the range of 10 to 1000 µgL-1, with a correction coefficient of 0.99. When spiked at 5, 100, and 500 µgkg-1, the recoveries ranged from 80.82% to 88.09%, with the intra-day and inter-day coefficients of variation from 10.76% to 13.48% and from 10.80% to 12.71%, respectively. The limit of quantification(LOQ) and detection(LOD) were 5.05 µg•mL-1 and 1.55 µgmL-1, respectively.
method:
It was extracted from the sample with 0.1% formic acid-methanol (10+90, v/v) and then purified on a hydrophilic-lipophilic balance cartridge. Chromatography separation was performed on an RP-C18 column, and detection of hainanmycin was done by positive electrospray ionization (ESI+) under the multiple reaction monitoring (MRM) mode.
Conclusion:
This method showed high sensitivity and accuracy for determining hainanmycin in chicken liver.
conclusion:
This method showed high sensitivity and accuracy for determining hainanmycin in chicken liver.
other:
nothing
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