The study was conducted to isolate and identify cellulose producing microorganisms from cassava waste water. Cassava waste water sample was obtained from a cassava processing factory at Lokogoma town in Wushishi Local government area of Niger State. The microorganisms were isolated, identified and counted by standard microbiological methods. The mean bacteria count ranges from 6.8 x 103 cfu/mL-1 to 2.1 x 103 cfu/mL-1 while the fungi count ranges from 3.2 x 103 cfu/mL-1 to 1.2 x 103 cfu/mL-1. A total of eight (8) bacterial; Staphylococcus aureus, Bacillus anthrax, Bacillus subtilis, Escherichia coli, Klebsilla sp, Bacillus megaterus, Staph. Epidermidis and Pseudomonas aeruginosa, and six (6) fungi; Saccharomyce serivicea, Aspergillus niger, Penecillium sp., Muccor sp., Aspergilus flavus and Aspergilus fumigetus isolates were identified in the waste water. Among these organism, the best cellulase activity was recorded for Bacillus subtilis (10.39 x 10-4 mg/ml/sec) and Aspergillus niger (11.21 x 10-4 mg/ml/sec). However, maximum activity was obtained at pH ranges from 3 ~ 9, temperature ranges from 30 oC ~ 80oC and substrate concentrations ranges from 1.5% ~3.0%. In conclusion, cassava processing water regarded as waste water could be an alternative source of microorganisms capable of producing cellulase enzyme for industrial purposes.
Proteolytic enzymes are ubiquitous in occurrence and find multiple applications in various industrial sectors. Although there are many microbial sources available for producing proteases, only a few are recognized as commercial producers. Utilization and recycling of renewable resources that pose threat to the environment can be systematically carried out to bring about resource productivity needed to make human activity sustainable. In the present study, we evaluated the phytochemical, antimicrobial, and protease production ability of mango seed kernel and pineapple peels. The proximate compositions and antimicrobial analysis of Mango seed kernel and pineapple peels were evaluated using standard protocols. We evaluated the protease production of Bacillus megaterium using the mango seed kernel and pineapple peels as the carbon sources. Our results revealed that mango seed kernel has low moisture, ash and crude fibre content but has high oil and crude protein content while pineapple peels have high moisture and fibre content but low in ash, crude protein and oil content. Mango seed extract also demonstrated antimicrobial activities against B. subtilis, less sensitive to B. megaterium and no activity against A. niger. However, the pineapple peel extracted is highly sensitive to B. subtilis and S aureus but demonstrated no activity against P. aeroginosa and A niger. The B. megaterium exhibited higher protease production ability when mango seed kernel was used as a carbon source at all tested concentrations. In conclusion, the information obtained from proximate and antimicrobial analysis of mango seed kernel and pineapple peels serves as a guide for the possible utilization as carbon sources for microbial enzyme production. Thus, both pineapple peel and mango seed kernel can be bio-remediated when used as carbon sources for protease production.
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