BackgroundHepatitis C virus (HCV) infection may cause liver diseases of various severities ranging from primary acute infection to life-threatening diseases, such as cirrhosis or hepatocellular carcinoma with poor prognosis. According to clinical findings, HCV infection may also lead to some extra-hepatic symptoms, including type 2 diabetes mellitus (DM). Since insulin resistance is the major etiology for type 2 DM and numerous evidences showed that HCV infection associated with insulin resistance, the involvement of E2 in the pathogenesis of type 2 DM and underlying mechanisms were investigated in this study.MethodsReverse transcription and real-time PCR, Western blot assay, Immunoprecipitation, Glucose uptake assay and analysis of cellular glycogen content.ResultsResults showed that E2 influenced on protein levels of insulin receptor substrate-1 (IRS-1) and impaired insulin-induced Ser308 phosphorylation of Akt/PKB and Ser9 phosphorylation of GSK3β in Huh7 cells, leading to an inhibition of glucose uptake and glycogen synthesis, respectively, and eventually insulin resistance.ConclusionsTherefore, HCV E2 protein indeed involved in the pathogenesis of type 2 DM by inducing insulin resistance.

To return a serve, one must pick up information from the server’s kinematics and anticipate the ball trajectory. Although the perceptual requirements are important, the literature diverges in terms of the differences between experts and novices as well as the importance of the experimental paradigm (in-situ vs. video-based) for the results. This study aimed to address both concerns. We compared experts’ (n=7, 20.6±1.1 year of age) and novices’ (n=7, 20.0±0.4 years of age) visual pattern when returning a serve (Experiment 1) and the influence of the experimental paradigm in experts (Experiment 2). Experts fixated more and longer the upper body and ball, while novices showed a more distributed pattern and with longer fixations outside of the server’s body. Also, the pattern was different when comparing in-situ and laboratory settings, differing mainly in fixation frequency. The influence of expertise was observed in qualitative (relative) and quantitative (absolute) measures of visual behavior with the setting having an important influence. Thus, studies should be as close to the actual situation if trying to understand experts’ behavior.

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