Fluorescence through proximity: Reduction‐triggered fluorescent probes bearing either a fluorescein derivative or triphenylphosphine bind sequence‐specifically at the branch site of lariat RNA. The chemical reaction between the two substrates leads to the formation of unquenched fluorescein and the emission of a fluorescence signal (see picture). Prelariat and lariat structures can be distinguished by using this method.
Fluoreszenz durch Nähe: Reduktionsinduzierte Fluoreszenzsonden, die ein Fluorescein‐Derivat oder Triphenylphosphan tragen, binden sequenzspezifisch am Verzweigungspunkt von Lariat‐RNA. Die chemische Reaktion der beiden Substrate führt zur Bildung von nativem Fluorescein und zur Emission eines Fluoreszenzsignals (siehe Bild). Prälariat‐ und Lariat‐Strukturen können mit dieser Methode unterschieden werden.
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