Detection of pre-mRNA splicing in vitro by an RNA-templated fluorogenic reaction

Tamura, Yasutsugu; Yoshimoto, Rei; Kawai, Yuto; Yoshida, Minoru; Tsuneda, Satoshi; Ito, Yoshihiro; Abe, Hiroshi

doi:10.1016/j.bmcl.2012.09.033

Cited by 12 publications

(10 citation statements)

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“…Another pioneering work has been done to detect the spliced mRNA; the RETF probes are designed to specifically target the splice junction site. 43 These two probes are unable to react as a template on the pre-mRNA because of the far distance between two exons, but they can react as a template on the spliced mRNA since that intron is removed and two exons are ligated together after splicing (Figure 3B). This method has demonstrated the capability of drug screening and flexibility for its easy application to any genes by simply altering the sequence of probes.…”

Section: In Vitro Analysis Of Pre-mrna Splicingmentioning

confidence: 99%

“…The probes are unable to react as a template on the pre-mRNA for the far distance between two exons, but the probes can react as a template on the spliced mRNA since that intron was removed and two exons were ligated together after splicing. Reprinted from Tamura et al 43 Copyright 2012 Elsevier. match between the 5 0 or 3 0 end of the boundary-spanning primer and that of the other splicing variants.…”

Section: Discrimination Among Different Alternative Splicing Variantsmentioning

confidence: 99%

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RNA Splicing Analysis: From In Vitro Testing to Single-Cell Imaging

Ren

Zhang

Deng

et al. 2019

Chem

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RNA splicing is a fundamental regulatory process of gene expression and plays a pivotal role in transcriptome complexity, cell-fate determination, and organism development. The detection of splicing products has attracted significant interest because aberrant splicing can lead to cell dysfunction and numerous diseases, such as cancer and neurodegeneration. Particularly, splicing variability between individual cells is primarily responsible for gene expression heterogeneity. Investigations into single-cell expression of RNA splicing variants offer a venue for resolving gene regulatory circuits, mapping intracellular localization, and classifying cell types. This review discusses the merits and technical challenges in transitioning RNA splicing detection from in vitro testing to single-cell analysis. In the end, we suggest some future directions for RNA splicing analysis and expect to inspire innovative works and discoveries in this field.

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Section: In Vitro Analysis Of Pre-mrna Splicingmentioning

confidence: 99%

Section: Discrimination Among Different Alternative Splicing Variantsmentioning

confidence: 99%

RNA Splicing Analysis: From In Vitro Testing to Single-Cell Imaging

Ren

Zhang

Deng

et al. 2019

Chem

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“…The templated reduction of azidorhodamine (Figure 15.1) was used to detect pre-mRNA spicing in vitro. [147] 2. In an extension of azide-based immolative linkers discussed in Section 4.7 (Figure 15.2), Shibata et al have succesfuly used a nucleic acid tempalted reaction to unmasks the function of a molecule that induce protein expression.…”

Section: Addendum (May 21 2013)mentioning

confidence: 99%

Reactions Templated by Nucleic Acids: More Ways to Translate Oligonucleotide‐Based Instructions into Emerging Function

2013

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The programmability of oligonucleotide recognition offers an attractive platform to direct the assembly of reactive partners that can engage in chemical reactions. Recently, significant progress has been made in both the breadth of chemical transformations and in the functional output of the reaction. Herein we summarize these recent progresses and illustrate their applications to translate oligonucleotide instructions into functional materials and novel architectures (conductive polymers, nanopatterns, novel oligonucleotide junctions); into fluorescent or bioactive molecule using cellular RNA; to interrogate secondary structures or oligonucelic acids; or a synthetic oligomer.

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“… Die Templat‐gestützte Reduktion von Azidorhodamin (Abbildung 15.1) wurde dazu verwendet, das Spleißen von prä‐mRNA in vitro zu detektieren 147 …”

Section: Addendum (21 Mai 2013)unclassified

Reaktionen an Nucleinsäuretemplaten: mehr Methoden zur Übersetzung Oligonucleotid‐basierter Informationen in neue Funktionen

Górska

Winssinger

2013

Angewandte Chemie

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Die Programmierbarkeit der Oligonucleotid‐Erkennung bietet eine vielversprechende Plattform, um die Anordnung von Reaktanten zu steuern. Sowohl bei der Bandbreite chemischer Umwandlungen als auch beim funktionalen Ergebnis der Reaktion wurden kürzlich erhebliche Fortschritte erzielt. Hier fassen wir diese jüngsten Fortschritte zusammen und erläutern ihre Anwendungen, z. B. die Übersetzung von Oligonucleotid‐Informationen in Funktionsmaterialien und neuartige Architekturen (leitende Polymere, Nanomuster, neuartige Oligonucleotid‐Kreuzungen) oder – mithilfe zellulärer RNA – in fluoreszierende oder bioaktive Moleküle; zur Untersuchung von Sekundärstrukturen oder Oligonucleinsäuren; sowie in ein synthetisches Oligomer.

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Detection of pre-mRNA splicing in vitro by an RNA-templated fluorogenic reaction

Cited by 12 publications

References 50 publications

RNA Splicing Analysis: From In Vitro Testing to Single-Cell Imaging

RNA Splicing Analysis: From In Vitro Testing to Single-Cell Imaging

Reactions Templated by Nucleic Acids: More Ways to Translate Oligonucleotide‐Based Instructions into Emerging Function

Reaktionen an Nucleinsäuretemplaten: mehr Methoden zur Übersetzung Oligonucleotid‐basierter Informationen in neue Funktionen

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