Food and beverages rich in polyphenols with antioxidant activity are highlighted as a potential factor for risk reduction of lifestyle related diseases. This study was conducted to elucidate total polyphenol consumption from beverages in Japanese people. Total polyphenol (TP) contents in beverages were measured using a modified Folin-Ciocalteu method removing the interference of reduced sugars by using reverse-phase column chromatography. A beverage consumption survey was conducted in the Tokyo and Osaka areas in 2004. Randomly selected male and female subjects (10-59 years old, n = 8768) recorded the amounts and types of all nonalcoholic beverages consumed in a week. Concentration of TP in coffee, green tea, black tea, Oolong tea, barley tea, fruit juice, tomato/vegetable juice, and cocoa drinks were at 200, 115, 96, 39, 9, 34, 69, and 62 mg/100 mL, respectively. Total consumption of beverages in a Japanese population was 1.11 +/- 0.51 L/day, and TP contents from beverages was 853 +/- 512 mg/day. Coffee and green tea shared 50% and 34% of TP consumption in beverages, respectively, and contribution of each of the other beverages was less than 10%. TP contents in 20 major vegetables and 5 fruits were 0-49 mg and 2-55 mg/100 g, respectively. Antioxidant activities, Cu reducing power, and scavenging activities for DPPH and superoxide, of those samples correlated to the TP contents (p < 0.001). Beverages, especially coffee, contributed to a large share of the consumption of polyphenols, as antioxidants, in the Japanese diet.

In previous paper,1] the average fitness index was developed as a mass-data comparison method, enabling the methylated sex pheromone of the varied carpet beetle Anthrenus vervasci2) to be reconfirmed as methyl 5-un-

Since all positional isomers of methyl undecenoates were available and also an undecenoate sex pheromone of the varied carpet beetle, identification of the double bond position of the pheromone was attempted by comparing the mass spectra of the isomers with that of the pheromone,~sing a newly devised' fitness index. The index (%) was calculated by dividing the absolute difference between the intensity ratio of every fragment ion-pair of an isomer and the corresponding ratio of the pheromone by the latter for each isomer. All the index values were summed up for each isomer and averaged to determine the similarity to the pheromone. The smallest average among the isomers indicates the best fit to the pheromone. The results of a comparison based on all commonly observable ions (a total of 63 ions above m/z 41, with intensities more than 0.1%) and 17 selected ion groups (above m/z 55 or m/z 59 with intensities of more than 0.1 %, 1%, 3%, 5%, 10% or 20%, respectively, and above m/z 41 with intensities more than 1%,3%,5%, 10% or 20%, respectively) indicated that methyl 5-undecenoate was the most probable candidate for the pheromone, except two ion groups (above m/z 41 with intensities of more than 10% and 20%, respectively). Furthermore, statistically significant results were obtained for all groups above m/z 41 with intensities up to 5% and groups above m/z 55 and 59 with intensities more than 10%. Therefore the present study verified the feasibility of using mass' spectrometry for the identification of the double bond position of a pheromone.

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