Objective: In the present study, the antidiabetic and antioxidant study of stem part of Coccinea grandis Voigt plant extracts in Streptozotocin induced diabetic rats were investigated. Materials and methods: Fifty four Wistar albino rats were used with nine groups and with six rats in each group. 45 mg/kg body weight streptozotocin was administered to group 2 to 9. Group 2 was diabetic control. Group 3 was given with glimepiride as standard drug. Group 4 and 5 were given petroleum ether extract 250 and 500 mg/kg respectively. Group 6 and 7 were given 250 and 500 mg/kg chloroform extract respectively. Group 8 and 9 were given 250 and 500 mg/kg hydro alcoholic extract respectively. Antidiabetic activity of the extracts was assessed by serum glucose level on glucose kit. Superoxide dismutase (SOD), Catalase (CAT) and lipid peroxidation studies were assessed with histopathology. Result: The chronic study data on diabetic rats cleared the administration of all extracts significantly reduced blood glucose level and lipid peroxidation level with better antioxidant activity. Conclusion: From the study, the petroleum ether, chloroform and hydro alcoholic extracts of stem part of Coccinea grandis Voigt plant have shown antidiabetic and antioxidant potential.
Key Words: Antidiabetic activity, antioxidant activity, Lipid peroxidation, Superoxide dismutase, Catalase.
Compound (9Z, 12Z)-octadeca-9, 12-dienoic acid (CGHY 02) was isolated from Coccinia grandis (L.) Voigt n-butanol extract by column chromatography. A molecular docking study against human pancreatic alpha-amylase enzyme (PDB: 3OLE) yielded a good docking score of -112.37 Kcal mol-1, indicating a high affinity of the compound to the receptor via hydrogen bonds, electrostatic interactions and hydrophobic interactions. The hydrogen bond was observed between the hydroxyl group of the compound and glutamine 8, whereas the carbonyl group and C5-C9 chain of the compound revealed steric interactions with glutamine 8 and aspartate 402, respectively. In vitro alpha-amylase inhibition of (CGHY 02) demonstrated remarkable inhibition (68.47 %) at low concentration (100 µL mL-1) compared to standard acarbose. Moreover, in silico ADME analysis of the compound exhibited 92.282 % gastrointestinal absorption, skin permeability, activity as a substrate for CYP2D6 and CYP3A4 enzymes resulting in better metabolism and 1.936 (logn ml min-1 kg-1) of total clearance of the compound. In silico toxicity predicted carcinogenicity, mutagenicity via PreADMET online server and hepatotoxicity and skin irritations via pkCSM online platforms. Antidiabetic potential of (CGHY 02) is attributed through inhibition of human pancreatic alpha amylase enzyme.
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