There is still a debate about the effectiveness of native globular proteins to perform as Pickering-like stabilizers for oil-in-water high internal phase emulsions (HIPEs). In the work, we report one native globular protein (ovalbumin) with strong structural integrity and high refolding ability, exhibits an outstanding Pickering stabilization for HIPEs. Ultrastable gel-like HIPEs can be formed through a facile one-pot homogenization even at a concentration as low as 0.2 wt %. The HIPEs formed in the protein-poor regime are a kind of self-supporting and remoldable hydrogel consisting of bridging droplets. The formed HIPEs also exhibit other unique characteristics, such as extraordinary coalescence stability (against prolonged storage or heating), susceptibility to freeze-thawing, enhanced oxidation stability (to encapsulated bioactives), and inhibited vaporization of volatile oils. The findings would be of importance for extending the HIPEs to be applied in food, cosmetic, and petroleum industries.
Aiming to achieve the modification to soy protein isolate (SPI) by soy soluble polysaccharides (SSPS), electrically driven complex systems were first established in the environment of pH 3.0, and then reconstituted SPI particles with different SPI-SSPS ratios were obtained under freeze-drying process. Through this treatment, the structures of SPI particles were partly unfolded and adsorbed SSPS mainly via hydrophobic interactions and hydrogen bonding with larger particle sizes. The adherence of SSPS decreased the surface hydrophobicity of reconstituted SPI particles, but exerted not much influence on the emulsifying and foaming activities and increased the corresponding stabilities due to enhancing the unfolded extent of structure and improving the conformation flexibility. Reconstituted SPI-SSPS particles might rearrange and link each other due to the presence of SSPS on the air-water interface to better stabilize these systems. At SPI-SSPS ratio of 10:1, lower temperature was required to form gels with lower gel intensity and porous structure. The findings provide a further comprehension to the relationship between structures and functional properties of SPI modified by SSPS and the feasibility of applying these reconstituted particles to needed areas.
The
postoperative tumor recurrence and chemotherapy resistance
in clinical osteosarcoma treatment have raised an imperative need
to develop local implants for selectively killing residual tumor cells
and simultaneously provide a scaffold for effectively filling the
tumor resection-induced bone defects. Herein, a multifunctional platform
is developed through successively coating TiN microparticles and doxorubicin
(DOX) on the surface of tricalcium phosphate (TCP) scaffolds to achieve
synergetic effects of photothermal therapy and chemotherapy for osteosarcoma.
The content of TiN and DOX in the scaffolds can be flexibly adjusted
by immersing the scaffolds into the solution containing different
concentrations of TiN and DOX. The excellent therapeutic effect was
achieved both in vitro and in vivo through the precise photothermal therapy and localized controlled-release
chemotherapy. Moreover, the overall bulk scaffolds provide the mechanical
support for bone tissue when implanting scaffolds into bone defects
resulting from surgical removal of osteosarcoma. Importantly, using
the poly(d,l-lactide) (PDLLA) as the medium, the
scaffolds can be exploited as a universal platform for loading different
kinds of therapeutic agents. This study may provide insights into
designing multifunctional local implantation for eradicating tumors
after surgical interventions with mitigated side effects.
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