In June 2013, excessive rainfall associated with an intense weather system triggered severe flooding in southern Alberta, which became the costliest natural disaster in Canadian history. This article provides an overview of the climatological aspects and large-scale hydrometeorological features associated with the flooding event based upon information from a variety of sources, including satellite data, upper air soundings, surface observations and operational model analyses. The results show that multiple factors combined to create this unusually severe event. The event was characterized by a slow-moving upper level low pressure system west of Alberta, blocked by an upper level ridge, while an associated well-organized surface low pressure system kept southern Alberta, especially the eastern slopes of the Rocky Mountains, in continuous precipitation for up to two days. Results from air parcel trajectory analysis show that a significant amount of the moisture originated from the central Great Plains, transported into Alberta by a southeasterly low level jet. The event was first dominated by significant thunderstorm activity, and then evolved into continuous precipitation supported by the synoptic-scale low pressure system. Both the thunderstorm activity and upslope winds associated with the low pressure system produced large rainfall amounts. A comparison with previous similar events occurring in the same region suggests that the synoptic-scale features associated with the 2013 rainfall event were not particularly intense; however its storm environment was the most convectively unstable. The system also exhibited a relatively high freezing level, which resulted in rain, rather than snow, mainly falling over the still snow-covered mountainous areas. Melting associated with this rain-on-snow scenario likely contributed to downstream flooding. Furthermore, above-normal snowfall in the preceding spring helped to maintain snow in the high-elevation areas, which facilitated the rain-on-snow event.
Abstract. This study quantifies the inconsistency in gauge precipitation observations across the border of Alaska and Yukon. It analyses the precipitation measurements by the national standard gauges (National Weather Service (NWS) 8 in. gauge and Nipher gauge) and the bias-corrected data to account for wind effect on the gauge catch, wetting loss and trace events. The bias corrections show a significant amount of errors in the gauge records due to the windy and cold environment in the northern areas of Alaska and Yukon. Monthly corrections increase solid precipitation by 136 % in January and 20 % for July at the Barter Island in Alaska, and about 31 % for January and 4 % for July at the Yukon stations. Regression analyses of the monthly precipitation data show a stronger correlation for the warm months (mainly rainfall) than for cold month (mainly snowfall) between the station pairs, and small changes in the precipitation relationship due to the bias corrections. Double mass curves also indicate changes in the cumulative precipitation over the study periods. This change leads to a smaller and inverted precipitation gradient across the border, representing a significant modification in the precipitation pattern over the northern region. Overall, this study discovers significant inconsistency in the precipitation measurements across the USA-Canada border. This discontinuity is greater for snowfall than for rainfall, as gauge snowfall observations have large errors in windy and cold conditions. This result will certainly impact regional, particularly cross-border, climate and hydrology investigations.
In herpesviruses, homologues of glycoprotein B (gB) are essential membrane proteins which are involved in fusion. However, there is no clear evidence regarding the location of the fusogenic domain on gB. By using bovine herpesvirus 1 (BHV-1) as a model, we studied the relationship between the structure and the fusogenic activity of gB. This was achieved by expressing genes of different gB derivatives containing specific truncations at the end of segments 2 or 3 of the transmembrane region in Madin-Darby bovine kidney cells under the control of the bovine heat-shock protein hsp70A gene promoter. All expressed gB products were structurally similar to authentic gB. One truncated form of gB, gBt, which contains residues 1-763, was efficiently secreted. However, gBtM (residues 1-807), which includes the first two segments at the carboxyl terminus, showed unstable retention on the cell surface, whereas gBtMA (residues 1 829), which contains all three membrane-spanning segments, was mostly intracellularly retained with some unstable surface anchorage. Another truncated gB, gBtDAF, which has gB residues 1-763 (gBt) and a human decay-accelerating factor (DAF) carboxyl tail, was also expressed. The DAF fragment provided a signal for the addition of a glycosyl phosphatidylinositol-based membrane anchor, which could target the gBt chimeric protein on the cell membrane. Immunofluorescence staining and pulse-chase kinetic studies support the theory that gBtM, gBtMA, and gBtDAF are retained on nuclear and cellular membranes via different segments of the transmembrane region or the DAF fragment, respectively. For the cells expressing gBt or gBtM, no cell fusion was observed, whereas cells expressing gBtMA clearly showed fusion. However, in gBtDAF cells, the overexpression and cellular accumulation of recombinant gB products did not cause fusion either, which supports our contention that the fusion phenomenon in gBtMA cells is caused by the fusogenic activity of the expressed gBtMA. With the help of sequence analysis, our results indicate that segment 2 of the transmembrane anchor region might be a fusogenic domain, whereas the real anchor is segment 3.
Data obtained from a variety of sources including, the Canadian Lightning Detection Network, weather radars, weather stations and operational numerical weather model analyses were used to address the evolution of precipitation during the June 2013 southern Alberta flood. The event was linked to a mid-level closed low pressure system to the west of the region and a surface low pressure region initially to its south. This configuration brought warm, moist unstable air into the region that led to dramatic, organized convection with an abundance of lightning and some hail. Such conditions occurred in the southern parts of the region whereas the northern parts were devoid of lightning. Initially, precipitation rates were high (extreme 15-min rainfall rates up to 102 mm h -1 were measured) but decreased to lower values as the precipitation shifted to longlived stratiform conditions. Both the convective and stratiform precipitation components were affected by the topography. Similar flooding events, such as June 2002, have occurred over this region although the 2002 event was colder and precipitation was not associated with substantial convection over southwest Alberta.
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