The present study aimed to investigate the biological function and underlying molecular mechanisms of miR-31 in osteoarthritis (OA). Reverse transcription-quantitative polymerase chain reaction was used to detect miR-31 expression, and it was found that miR-31 was downregulated in the cartilage tissues of OA patients.
was used to predict the gene targets of miR-31, and dual luciferase reporter assays were used to verify that C-X-C motif chemokine ligand 12 (CXCL12) was a direct target of miR-31. The human chondrocyte cell line CHON-001 was used to perform MTT and cell migration assays. Western blotting was used to measure the protein expression of CXCL12, type I collagen and aggrecan. The results suggested that CXCL12 was a target of miR-31, and the expression of CXCL12 was negatively regulated by miR-31 in CHON-001 cells. miR-31 increased CHON-001 cell viability and migration, as well as the expression of type I collagen and aggrecan. Furthermore, the overexpression of CXCL12 eliminated the effects of miR-31 mimics on CHON-001 cells. In conclusion, the data indicated that miR-31 promoted chondrocyte viability and migration by directly targeting CXCL12, which provided evidence for CXCL12 as a potential target in OA therapy.
A retrospective cohort study to explore the clinical efficacy and safety evaluation of calcitriol combined with bisphosphonates in the therapy of postmenopausal osteoporosis is conducted. The postmenopausal osteoporosis sufferers admitted to our hospital from January 2020 to June 2021 are retrospectively collected and divided into a contrast set and a study set, with 60 cases in each set. For the contrast set, all sufferers are treated with bisphosphonates. For the study set, on the basis of the therapy drugs in the contrast set, they are treated with calcitriol capsules. Firstly, the curative effects, bone mineral density standards, and bone metabolism standards of the two sets are contrasted; then, the lumbar spine bone mineral density, VAS score, and quality of life between the two sets of sufferers before therapy and 1 year after therapy are contrasted, and the correlation between bone mineral density and VAS and quality of life of the sufferers is analyzed. Lastly, the readmission situation between the two sets at one year is contrasted. The experimental results show that for postmenopausal women with osteoporosis, calcitriol combined with bisphosphonate therapy can notoriously enhance the clinical therapy effect of sufferers, with low adverse reactions, and can effectively enhance the bone mineral density and bone density of sufferers.
Objective: Construction of a novel biomimetic nano-structured multi-layer interface implant to induce osseointegration. Methods: Spark plasma sintering was used to create micron-scale trabecular bone biomimicry structures, surface modification with TiO2 nanotubes,
and micron-scale trabecular bone biomimicry/TiO2 nanotubes from pure titanium samples. The structure of the modified surface. Four groups (A, B, C, and D) of 40 female SD rats were randomly divided into each of the four groups and implanted with titanium, trabecular bone biomimetic
titanium, TiO2 nanotube surface modified titanium, and micron trabecular bone biomimetic/TiO2 nanotube surface modified trabecular titanium. Results: The proximal tibia and titanium implants underwent histological, micro-CT, and biomechanical testing after 12 weeks.
Conclusions: The best osseointegration was shown in Group D, which had the greatest osseointegration rate, relative bone volume, and total bone volume, as well as greater trabecular bone-related measures. It was also shown that group D had the highest maximum pushing force and critical
shear strength compared to the other three groups. Conclusion: Ultimately, the biomimetic nano-structured multi-layer implant improves the osseointegration of titanium implants, making it a suitable material for use in clinical therapy.
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