The ripening of papaya is a physiological and metabolic process associated with accumulation of carotenoids, alternation of flesh color and flavor, which depending on genotype and external factors such as light and hormone. Transcription factors regulating carotenoid biosynthesis have not been analyzed during papaya fruit ripening. RNA-Seq experiments were implemented using different ripening stages of papaya fruit from two papaya varieties.
Cis
-elements in lycopene β-cyclase genes (
CpCYC-B
and
CpLCY-B
) were identified, and followed by genome-wide analysis to identify transcription factors binding to these
cis
-elements, resulting in the identification of
CpbHLH1
and
CpbHLH2
, two bHLH genes. The expressions of
CpbHLH1/2
were changed during fruit development, coupled with transcript increase of carotenoid biosynthesis-related genes including
CpCYC-B
,
CpLCY-B
,
CpPDS2
,
CpZDS
,
CpLCY-E
, and
CpCHY-B
. Yeast one-hybrid (Y1H) and transient expression assay revealed that
CpbHLH1/2
could bind to the promoters of
CpCYC-B
and
CpLCY-B
, and regulate their transcriptions. In response to strong light, the results of elevated expression of carotenoid biosynthesis-related genes and the changed expression of
CpbHLH1/2
indicated that
CpbHLH1/2
were involved in light-mediated mechanisms of regulating critical genes in the carotenoid biosynthesis pathway. Collectively, our findings demonstrated several TF family members participating in the regulation of carotenoid genes and proved that
CpbHLH1
and
CpbHLH2
individually regulated the transcription of lycopene β-cyclase genes (
CpCYC-B
and
CpLCY-B
). This study yielded novel findings on regulatory mechanism of carotenoid biosynthesis during papaya fruit ripening.
In this study, nanoscale zero-valent iron (NZVI) was synthesized by conventional liquid-phase chemical reduction methods without a support material and then characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM) and X-ray diffraction (XRD). The effect of NZVI particles on phosphate removal from aqueous solutions was examined. The results showed that the phosphate removal efficiency increased from 34.49% to 87.01% as the dosage of nanoscale iron particles increased from 100 to 600 mg L(-1) with an initial phosphate concentration of 10 mg L(-1), and the phosphate removal efficiency decreased from 72.89% to 51.39% as the initial phosphate concentration increased from 10 to 90 mg L(-1), with 400 mg L(-1) NZVI. Phosphate removal efficiencies of 99.41% and 95.09% were achieved at pH values of 2 and 4, respectively, with an initial phosphate concentration of 20 mg L(-1) and 400mg L(-1) NZVI. The use of NZVI particles synthesized in a carboxymethyl cellulose (CMC)-water solution significantly enhanced phosphate removal from an aqueous solution compared with the use of NZVI synthesized in an ethanol-water solution. NZVI particles achieved 71.34% phosphate removal, which was remarkably higher than that of microscale zero-valent iron (MZVI) particles (16.35%) with 10 mg L(-1) of phosphate and 400mg L(-1) iron. Based on the removal mechanism analysis performed in this study, we recommend that phosphate removal be accomplished by simultaneous adsorption and chemical precipitation. The XRD patterns of the NZVI before and after the reactions indicated the formation of crystalline vivianite (Fe3(PO4)2 x 8H2O) during the procedure.
A new series of flavonoid derivatives have been designed, synthesised and evaluated as acetylcholinesterase inhibitors that could bind simultaneously to the peripheral and catalytic sites of the enzyme. Among them, fifteen derivatives were found to inhibit the enzyme in the micromolar range and isoflavone derivatives possessed more potent inhibitory activity than other flavonoid derivatives. The best compound 9a had its inhibitory activity (IC 50 ¼ 0.093mM) in the same range as the reference compound, donepezil (IC 50 ¼ 0.025mM). Preliminary structure-activity relationships and a molecular modeling study for 9a have revealed that the isoflavone moiety plays a key role in the interaction of this series of derivatives with AChE by acting as an anchor in its peripheral anionic site.
For the equilibrium immiscible Cu-Co system with a positive heat of formation of +10 kJ mol −1 , ab initio calculations were used to predict the physical properties of the metastable D0 19 and L1 2 structures for the Cu 75 Co 25 phases and the D0 19 structure for the Cu 25 Co 75 alloy. Based on the ab initio calculation results, an n-body Cu-Co potential was constructed and proven to be realistic. Applying the constructed Cu-Co potential, molecular dynamics simulations predict that the amorphous phase could be obtained at around Cu 60 Co 40 and its atomic distribution could be inhomogeneous. Experimentally, by using ion beam mixing with 200 keV Xe + ions, an amorphous Cu 60 Co 40 phase with inhomogeneous morphology was indeed obtained at a dose of 1 × 10 15 Xe + cm −2 . Increasing the irradiation dose to 4 × 10 15 Xe + cm −2 , a mixture of Cu-rich and Co-rich metastable phases was obtained. Besides, a mixture of FCC and HCP structures was observed in the Cu 82 Co 18 multilayered sample and an HCP structure was observed in the Cu 26 Co 74 multilayered sample. It was found that the lattice constants of the FCC and HCP phases determined by diffraction analysis were quite compatible with those predicted by the ab initio calculations.
Since a large number of contaminants are detected in source waters (SWs) and tap waters (TWs), it is important to perform a comprehensive effect evaluation and key contributor identification. A reduced human transcriptome (RHT)-based effect-directed analysis, which consisted of a concentrationdependent RHT to reveal the comprehensive effects and noteworthy pathways and systematic identification of key contributors based on the interactions between compounds and pathway effects, was developed and applied to typical SWs and TWs along the Yangtze River. By RHT, 42% more differentially expressed genes and 33% more pathways were identified in the middle and lower reaches, indicating heavier pollution. Hormone and immune pathways were prioritized based on the detection frequency, sensitivity, and removal efficiency, among which the estrogen receptor pathway was the most noteworthy. Consistent with RHT, estrogenic effects were widespread along the Yangtze River based on in vitro evaluations. Furthermore, 38 of 100 targets, 39 pathway-related suspects, and 16 estrogenic nontargets were systematically identified. Among them, diethylstilbestrol was the dominant contributor, with the estradiol equivalent (EEQ) significantly correlated with EEQ water . In addition, zearalenone and niclosamide explained up to 54% of the EEQ water . The RHT-based EDA method could support the effect evaluation, contributor identification, and risk management of micropolluted waters.
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