siRNA‐mediated management of myocardial ischemia reperfusion (IR) injury is greatly hampered by the inefficient myocardial enrichment and cardiomyocyte transfection. Herein, nanocomplexes (NCs) reversibly camouflaged with a platelet–macrophage hybrid membrane (HM) are developed to efficiently deliver Sav1 siRNA (siSav1) into cardiomyocytes, suppressing the Hippo pathway and inducing cardiomyocyte regeneration. The biomimetic BSPC@HM NCs consist of a cationic nanocore assembled from a membrane‐penetrating helical polypeptide (P‐Ben) and siSav1, a charge‐reversal intermediate layer of poly(l‐lysine)‐cis‐aconitic acid (PC), and an outer shell of HM. Due to HM‐mediated inflammation homing and microthrombus targeting, intravenously injected BSPC@HM NCs can efficiently accumulate in the IR‐injured myocardium, where the acidic inflammatory microenvironment triggers charge reversal of PC to shed off both HM and PC layers and allow the penetration of the exposed P‐Ben/siSav1 NCs into cardiomyocytes. In rats and pigs, BSPC@HM NCs remarkably downregulates Sav1 in IR‐injured myocardium, promotes myocardium regeneration, suppresses myocardial apoptosis, and recovers cardiac functions. This study reports a bioinspired strategy to overcome the multiple systemic barriers against myocardial siRNA delivery, and holds profound potential for gene therapy against cardiac injuries.
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Nowadays phone distraction has started to become an increasingly recognized phenomenon. This article aims to examine the influences of phone use on pedestrian crossing behavior at signalized intersections in China. Using video recording and manual counting, pedestrian crossing behavior, age, gender, phone use, and waiting time are obtained at four signalized intersections. Totally, 4196 pedestrians are observed in four peak hours. Among them, 328 pedestrians (7.82%) are using their mobile phones, including 162 male pedestrians and 166 female pedestrians. The average phone use rate in different age groups are presented as follows: children (4.49%), youth (10.69%), middle-aged (6.87%), and elderly (1.15%). In terms of the phone using behavior on the crosswalk, age is a significant factor, while gender is not. For the type of violation behavior, the results show that pedestrians who use mobile phones are most likely to be late starters. In addition, some other important results are found: mobile phone use can raise the violation of pedestrian crossing behavior; pedestrians using their phones are more likely to cross on red; and pedestrians using their phones while crossing walk more slowly. Finally, the application significance of this study and some recommendations are provided to improve pedestrian safety.
In this paper, based on directional modulation (DM), robust beamforming matrix design for sum secrecy rate maximization is investigated in multi-user systems. The base station (BS) is assumed to have the imperfect knowledge of the direction angle toward each eavesdropper, with the estimation error following the Von Mises distribution. To this end, a Von Mises distribution-Sum Secrecy Rate Maximization (VMD-SSRM) method is proposed to maximize the sum secrecy rate by employing semi-definite relaxation and first-order approximation based on Taylor expansion to solve the optimization problem. Then in order to optimize the sum secrecy rate in the case of the worst estimation error of direction angle toward each eavesdropper, we propose a maximum angle estimation error-SSRM (MAEE-SSRM) method. The optimization problem is constructed based on the upper and lower bounds of the estimated eavesdropping channel related coefficient and then solved by the change of the variable method. Simulation results show that our two proposed methods have better sum secrecy rate than zero-forcing (ZF) method and signal-to-leakage-andnoise ratio (SLNR) method. Furthermore, the sum secrecy rate performance of our VMD-SSRM method is better than that of our MAEE-SSRM method.
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