A method to determine alkylphenols in Ginkgo biloba sarcotesta, especially focusing on polyunsaturated ginkgolic acid components, was developed on the basis of thermochemolysis-gas chromatography/mass spectrometry with a DB-23 column in the presence of trimethylsulfonium hydroxide [(CH 3 ) 3 SOH]. By using this technique, powder sample of Ginkgo biloba sarcotesta can be directly analyzed without any tedious and time-consuming pretreatment. On the resulting chromatograms, seven kinds of ginkgolic acids including polyunsaturated ginkgolic acid GA17:2 and two kinds of ginkgols were clearly observed as their methyl derivative. Based on the peak areas, the ginkgolic acid compositions are in good agreement with these obtained by a high-performance liquid chromatography method dedicated to analysis of ginkgolic acid.
A simple method was established for the determination of β-propiolactone (BPL) in human inactivated rabies vaccine by gas chromatography-mass spectrometry (GC-MS). The determination was performed on an Agilent HP-INNOWAX (30 m × 0.32 mm i.d., 0.25 µm) capillary column at the temperature of 80 °C. Electrospray ionization (ESI) was used by selective ion detection at m/z 42. The temperature for ESI source and inlet was set at 230 °C and 200 °C, respectively. Helium was used as the carrier gas at a flow rate of 25.1 mL/min. The total run time was 8 min. Acetonitrile and other components in the sample did not interfere with the determination of BPL. The results showed good linearity of BPL in the range of 0.50–10.01 μg/mL, with the limit of detection and the limit of quantification of 0.015 μg/mL and 0.050 μg/mL, respectively. Satisfactory precision was achieved for the current developed method. The method was applied to detect 6 batches of vaccine samples, and the results indicated that the target analyte BPL was present in three batches of unpurified samples, but was not detected in the purified samples, indicating the test samples were qualified. The established method was proved to be simple, versatile and sensitive, which can meet the requirements of quality control of BPL in human inactivated rabies vaccine.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.