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48sulfide used by its symbionts. δ 15 N tissue values differed between the mussels, with B. 49 platifrons having a wider range of on average slightly lower values (mean = 50 3 +0.5±0.7‰, n=36) than B. aduloides (mean = +1.1±0.0‰). These values are 51 significantly lower than δ 15 N values of South China Sea deep-sea sediments (+5‰ to 52 +6‰), indicating that the organic nitrogen is of local origin, possibly resulting from the 53 activity of autotrophic bacteria and due to assimilation of isotopically light nitrate or 54 ammonium by the symbionts. 55 56

Trends of annual and monthly temperature, precipitation, potential evapotranspiration and aridity index were analyzed to understand climate change during the period 1971-2000 over the Tibetan Plateau which is one of the most special regions sensitive to global climate change. FAO56-Penmen-Monteith model was modified to calculate potential evapotranspiration which integrated many climatic elements including maximum and minimum temperatures, solar radiation, relative humidity and wind speed. Results indicate generally warming trends of the annual averaged and monthly temperatures, increasing trends of precipitation except in April and September, decreasing trends of annual and monthly potential evapotranspiration, and increasing aridity index except in September. It is not the isolated climatic elements that are important to moisture conditions, but their integrated and simultaneous effect. Moreover, potential evapotranspiration often changes the effect of precipitation on moisture conditions. The climate trends suggest an important warm and humid tendency averaged over the southern plateau in annual period and in August. Moisture conditions would probably get drier at large area in the headwater region of the three rivers in annual average and months from April to November, and the northeast of the plateau from July to September. Complicated climatic trends over the Tibetan Plateau reveal that climatic factors have nonlinear relationships, and resulte in much uncertainty together with the scarcity of observation data. The results would enhance our understanding of the potential impact of climate change on environment in the Tibetan Plateau. Further research of the sensitivity and attribution of climate change to moisture conditions on the plateau is necessary.

This paper describes the general moisture conditions and the annual and seasonal trends of temperature, precipitation, potential evapotranspiration and aridity/humidity index from 1971 to 2000 in China. Observed climatic data from 616 meteorological stations over China's land surface were used. Potential evapotranspiration was calculated by the Penman-Monteith model with a modified net radiation part to accommodate China's unique climatic conditions. According to the aridity/humidity index, a ratio of potential evapotranspiration to precipitation, four moisture regional types were delineated gradually from the southeast to the northwest, i.e. humid, subhumid, semiarid and arid throughout China. Linear regression was performed on the 30-year time series of the four climate factors in order to detect possible trends. The results confirm the obvious spatial and temporal difference of climate trends. Surface air temperature has increasing trends almost all over China especially in winter. Most stations are statistically significant. Annual precipitation shows increasing trends at more than half of the stations, and the increasing trends mainly occur in summer and winter while the decreasing trends occur in spring and autumn. However, most stations are not statistically significant. Potential evapotranspiration has decreasing trends in most stations and nearly half of the stations are statistically significant. Annual aridity/humidity index shows primarily decreasing trends with a distribution nearly the same as the increasing trends of precipitation. The index has the decreasing trends in spring and summer and the increasing trends in autumn and winter. The results suggest that it is necessary to consider precipitation and potential evapotranspiration simultaneously to describe the moisture conditions exactly.

When its hydrothermal supply ceases, hydrothermal sulfide chimneys become inactive and commonly experience oxidative weathering on the seafloor. However, little is known about the oxidative weathering of inactive sulfide chimneys, nor about associated microbial community structures and their succession during this weathering process. In this work, an inactive sulfide chimney and a young chimney in the early sulfate stage of formation were collected from the Main Endeavor Field of the Juan de Fuca Ridge. To assess oxidative weathering, the ultrastructures of secondary alteration products accumulating on the chimney surface were examined and the presence of possible Fe-oxidizing bacteria (FeOB) was investigated. The results of ultrastructure observation revealed that FeOB-associated ultrastructures with indicative morphologies were abundantly present. Iron oxidizers primarily consisted of members closely related to Gallionella spp. and Mariprofundus spp., indicating Fe-oxidizing species likely promote the oxidative weathering of inactive sulfide chimneys. Abiotic accumulation of Fe-rich substances further indicates that oxidative weathering is a complex, dynamic process, alternately controlled by FeOB and by abiotic oxidization. Although hydrothermal fluid flow had ceased, inactive chimneys still accommodate an abundant and diverse microbiome whose microbial composition and metabolic potential dramatically differ from their counterparts at active vents. Bacterial lineages within current inactive chimney are dominated by members of α-, δ-, and γ-Proteobacteria and they are deduced to be closely involved in a diverse set of geochemical processes including iron oxidation, nitrogen fixation, ammonia oxidation and denitrification. At last, by examining microbial communities within hydrothermal chimneys at different formation stages, a general microbial community succession can be deduced from early formation stages of a sulfate chimney to actively mature sulfide structures, and then to the final inactive altered sulfide chimney. Our findings provide valuable insights into the microbe-involved oxidative weathering process and into microbial succession occurring at inactive hydrothermal sulfide chimney after high-temperature hydrothermal fluids have ceased venting.

Terpene cyclases (TCs), extraordinary enzymes that create the structural diversity seen in terpene natural products, are traditionally divided into two classes, class I and class II. Although the structural and mechanistic features of class I TCs are well-known, the corresponding details in class II counterparts have not been fully characterized. Here, we report the genome mining discovery and structural characterization of two class II sesquiterpene cyclases (STCs) from Streptomyces. These drimenyl diphosphate synthases (DMSs) are the first STCs shown to possess β,γ-didomain architecture. High-resolution X-ray crystal structures of DMS from Streptomyces showdoensis (SsDMS) in complex with both a farnesyl diphosphate and Mg2+ unveiled an induced-fit mechanism, with an unprecedented Mg2+ binding mode, finally solving one of the lingering questions in class II TC enzymology. This study supports continued genome mining for novel bacterial TCs and provides new mechanistic insights into canonical class II TCs that will lead to advances in TC engineering and synthetic biology.

Using a culture-independent method that combines CARD-FISH, qPCR and 16S rDNA, we investigated the abundance, community structure and diversity of microbes along a steep thermal gradient (50–90 °C) in the Tengchong Geothermal Field. We found that Bacteria and Archaea abundance changed markedly with temperature changes and that the number of cells was lowest at high temperatures (90.8 °C). Under low-temperature conditions (52.3–74.6 °C), the microbial communities were dominated by Bacteria, which accounted for 60–80% of the total number of cells. At 74.6 °C, Archaea were dominant, and at 90.8 °C, they accounted for more than 90% of the total number of cells. Additionally, the microbial communities at high temperatures (74.6–90.8 °C) were substantially simpler than those at the low-temperature sites. Only a few genera (e.g., bacterial Caldisericum, Thermotoga and Thermoanaerobacter, archaeal Vulcanisaeta and Hyperthermus) often dominated in high-temperature environments. Additionally, a positive correlation between Ammonia-Oxidizing Archaea (AOA) activity and temperature was detected. AOA activity increased from 17 to 52 pmol of NO2− per cell d−1 with a temperature change from 50 to 70 °C.

The impact of hydrothermal activity on wider ocean geochemistry and microbial ecology