The design and synthesis of two imidazole fluorescent probes for the special recognition of HClO/NaHSO3 and their applications are shown.

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In this paper, two dual function fluorescent probes P1 and P2 with obvious fluorescence spectrum change were developed for the detection of Zn2+ and H2S. Density functional theory (DFT) was employed to investigate the detection mechanism. A further illustration of the fluorescence quenching mechanism was also studied by 1H NMR titration and UV‐Vis absorption spectroscopy. Both probes are new compounds containing coumarin structure as fluorophore. They can quantitatively detect Zn2+ and H2S in the concentration range from 0 to 50 μM with excellent selectivity and anti‐interference ability over many metal ions or anions. Most worthy is that probes P1 and P2 have better cell permeability with lower cytotoxicity, which can be used for fluorescence detection in MCF‐7 cells, as well as the detection of exogenous Zn2+ in organisms.

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