Confocal laser scanning microscopy is an essential analytical tool in biological, biomedical, and material sciences, integrating microscope manufacturing technology, optical-electronic technology, and computer technology. In the last decade, confocal laser scanning microscopy has been successfully applied to the study of amber bioinclusions. Enhanced signal to noise ratios, resolution power, capability of optical sectioning, three-dimensional reconstruction, and better performance when imaging thicker samples provide a great deal of valuable and detailed morphological information about amber fossils. We briefly discuss the practical applications of CLSM in amber studies and compare it with other imaging methods commonly used in the field, including bright-field microscopy, wide-field fluorescence microscopy, and micro-computed tomography. A general procedure for imaging amber inclusions with CLSM is provided, with a focus on pretreatments and image processing.
Recent findings of ommatids from mid-Cretaceous Burmese amber have greatly increased our knowledge on the Mesozoic diversity of Ommatidae. Here, we report the first distinctly miniaturized ommatid species, Miniomma chenkuni gen. et sp. nov., entombed in mid-Cretaceous amber from northern Myanmar. This new fossil species is characterized by its small body size (less than 2 mm long) and subglobular metacoxae. Our discovery of miniaturization in extinct Ommatidae suggests a high ecological diversity of this family in the Mesozoic.
Ptiliidae is a group of distinctly miniaturized staphylinoid beetles with a scarce fossil record. Here, we report a new ptiliid genus and species, Crenossidium slipinskii Li, Newton and Cai gen. et sp. nov., from mid-Cretaceous amber from northern Myanmar. Crenossidium can be attributed to the subfamily Nossidiinae based on the hind wing morphology, which has also been confirmed through phylogenetic analyses. Crenossidium differs from other extant nossidiine genera in the combination of the wide apical maxillary palpomeres, posteriorly widest pronotal disk, (almost) contiguous procoxae, fewer setae along wing margin, and multidentate pygidium.urn:lsid:zoobank.org:pub:8038D763-6856-4AC5-972C-E20D636137EE.
We here report a new elateroid, Anoeuma lawrencei Li, Kundrata and Cai gen. et sp. nov., from mid-Cretaceous Burmese amber. Though superficially similar to some soft-bodied archostematans, Anoeuma could be firmly placed in the polyphagan superfamily Elateroidea based on the hind wing venation. Detailed morphological comparisons between extant elateroids and the Cretaceous fossils suggest that the unique character combination does not fit with confidence into any existing soft-bodied elateroid group, although some characters indicate possible relationships between Anoeuma and Omalisinae. Our discovery of this new lineage further demonstrates the past diversity and morphological disparity of soft-bodied elateroids.
Variegated mud-loving beetles, or Heteroceridae, are a small family belonging to the polyphagan superfamily Byrrhoidea. To date, only two poorly preserved compression fossils have been known from the Early Cretaceous of Eurasia. Here we describe the first heterocerid beetles in mid-Cretaceous amber from northern Myanmar, Excavotarsus lini gen. et sp. nov. and Ex. minor sp. nov.. The two new fossil species are distinguished from all extant heterocerids by their elongate body shape, apical 8–9 antennomeres forming a loose serrate club, pronotum longer than wide, protibia lacking robust spines, and two-segmented protarsi. This peculiar combination of plesiomorphic and derived characters suggests that Excavotarsus represents an early-diverging lineage of Heteroceridae and indicates that the family originated and diversified before the mid-Cretaceous.
The third member of the extinct ommatid genus Paraodontomma is reported from mid-Cretaceous Burmese amber. Our observation confirms the transverse ridges on elytra as a diagnostic character for the genus. Paraodontomma leptocristatum sp. nov. differs from previously reported congeners mainly in head subquadrate and without prominent protuberances, pronotal disc without prominent ridges, elytral ridges indistinct, and teeth along elytral margins not forming a wavy pattern. Musculature is preserved in the newly discovered specimen of P. leptocristatum, which further demonstrates the preservation potential and irreplaceable value of amber fossils.
Multi‐fold refractive seismic exploration is such a method that is generally used to trace the geological structural interfaces when the reflected seismic section is not good or used to trace the base structural configuration and to study the base interface. This paper will discuss the theory and method of imaging underground refractors with the refractied seismic waves in the refractied seismic data, based on the refraction theory. The method was called common depth refraction segments stack imaging method. The velocity analysis steps and NMO processing of the refractive wave by use of the theory and the method will also be given. A better imaging method, the common depth refractive point stack imaging method, will be proposed in the paper. Finally, the exactness and the practicality of the given methods will be verifired by processing model example and real data.
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