Vγ9Vδ2 T cells are promising candidates for cellular tumor immunotherapy. Due to their HLA-independent mode of action, allogeneic Vγ9Vδ2 T cells can be considered for clinical application. To apply allogeneic Vγ9Vδ2 T cells in adoptive immunotherapy, the methodology used to obtain adequate cell numbers with optimal effector function in vitro needs to be optimized, and clinical safety and efficacy also need to be proven. Therefore, we developed a novel formula to improve the expansion of peripheral γδ T cells from healthy donors. Then, we used a humanized mouse model to validate the therapeutic efficacy of expanded γδ T cells in vivo; furthermore, the expanded γδ T cells were adoptively transferred into late-stage liver and lung cancer patients. We found that the expanded cells possessed significantly improved immune effector functions, including proliferation, differentiation, and cancer cell killing, both in vitro and in the humanized mouse model. Furthermore, a phase I clinical trial in 132 late-stage cancer patients with a total of 414 cell infusions unequivocally validated the clinical safety of allogeneic Vγ9Vδ2 T cells. Among these 132 patients, 8 liver cancer patients and 10 lung cancer patients who received ≥5 cell infusions showed greatly prolonged survival, which preliminarily verified the efficacy of allogeneic Vγ9Vδ2 T-cell therapy. Our clinical studies underscore the safety and efficacy of allogeneic Vγ9Vδ2 T-cell immunotherapy, which will inspire further clinical investigations and eventually benefit cancer patients.

The TFF, in concentration mode at TMP of 10 psi, is a relatively quick, efficient, and cost-effective technique for purification and concentration of a large nanoparticle batch (>or=200 ml). The DCD technique can be an alternative purification method for nanoparticle dispersions of small volumes.

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The improvement of the gene distribution within skeletal muscle to enhance the immunogenicity of DNA vaccines is reported. Magnetic force acting on DNA vaccine associated with magnetic nanoparticles (see image), the major potential of which lies in extraordinarily rapid and efficient transfection at low vector doses, is utilized. After immunization, the magnetic DNA vector induces significantly enhanced serum antibody responses 2–3 orders of magnitude greater than naked DNA.

Metal-organic frameworks (MOFs) have been regarded as an ideal material for the development of functional textiles with filtration function. Such functional textiles with filtration function can be further used to develop personal protective equipment, such as protective masks. This paper focuses on the comparisons of different processes when applying MOFs to conventional textiles. Two different processes existing in the literature, namely the electrospinning method and hot-pressing method, are discussed in this paper. Materials loaded with MOFs developed with these two processes are evaluated and compared, regarding the adsorption of dyes in water and the removal of pollutants. Experiment results indicate that the hot-pressing method is more advantageous when applying MOF to textiles, in terms of adsorption and removal efficiency.

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