BACKGROUND:Stroke is a leading cause of disability and remains the second leading cause of death in the world. Some of the pathogenesis of stroke are interactions between genetic and acquired risk factors, the interaction is related with the atherosclerotic which is the main pathogenesis of ischaemic stroke. Previous studies demonstrated an association between methylene tetra hydro folate reductase (MTHFR) genotype and ischaemic stroke; the MTHFR C677T genotype is one of the independent risk factor.AIM:This study aims to know about the role of polymorphism gen MTHFR C677T in ischaemic stroke patients with and without hypertension.METHODS:This study is a cross-sectional study, the sample was taken consecutively, after approval by the Medical Faculty Science’s Ethics Committee at University Sumatera Utara. All sample matched with inclusion and exclusion criteria, demography data and blood sample were taken. Demography data were analysed using descriptive statistic.RESULTS:Of the 106 ischaemic stroke patients were divided into two groups, the first group is patients with hypertension (53 patients), and the second group is without hypertension (53 patients). We have done the PCR- RFLP to all the patients, we got 78 patients with 677CC of MTHFR genotype, 23 patients with 677CT genotype and 5 patients with 677TT genotype. We found polymorphism C677T is more frequent in ischaemic stroke patients with hypertension (16 patients; 69.5%), and all the patient with 677TT genotype are an ischaemic stroke with hypertension (5 patients; 100%).CONCLUSION:We concluded that polymorphism MTHFR C677T have an important role in hypertension and ischaemic stroke.
Background: Psoriasis is a chronic inflammation of the skin caused by combination of genetic, immune and environmental factors. The Vitamin D receptors alongside with plasma vitamin D (25(OH)D) level have known to be related with psoriasis. Vitamin D receptor polymorphism is one of the multiple polymorphisms that predispose individuals to several diseases. It is possible that this polymorphism is different among psoriatic patients and healthy one particularly in Medan, Indonesia population. Objective: We aimed to investigate associations between plasma level of 25(OH)D and one VDR gene polymorphism (A-1012G) with psoriasis.Methods: Fourty four psoriatic patients and 44 healthy control subjects’ DNA samples were obtained in this case control study and genotyped for A-1012G polymorphism by Polymerase Chain Reaction (PCR). The plasma vitamin D (25(OH)D) level of case and control subjects were examined using Enzyme Linked Immunosorbent Assay (ELISA).Results: Significant lower plasma 25(OH)D levels were found in control group (p<0.001) which consist of mostly young adult female. There is no significant relationship between AA, AG and GG genotype variances of A-1012G polymorphism with psoriasis (p=0.124).Conclusion: No significant association found between A-1012G polymorphism and psoriasis but there was a significant difference found between vitamin D level and psoriasis (p<0.001). From this study, vitamin D deficiency were more common in young adult female
ABSTRAK
ABSTRACTBackground: Neutropenia is the most common adverse event of breast cancer chemotherapy which can be life threatening due to opportunistic infection, neutropenic episodes may lead to delay or reduction of drug doses which may compromise treatment outcomes. In this study, we investigated the association of ATP-binding cassette subfamily B member 1 (ABCB1) gene C3435T polymorphism with the grading of neutropenia in breast cancer patients who treated with doxorubicin-taxan.
Objective: To analyze the ability of saliva in controlling the growth and the biofilm formation of Streptococcus mutans (S. mutans) as well as the effect of histatin-5 anti-biofilm relate to pH and saliva viscosity. Material and Methods: The S. mutans biofilm assayed by crystal violet 1% and its growth measured by spectrophotometer. The saliva viscosity was analyzed by viscometer, and pH of saliva was measured by pH meter. Results: Based on the optical density values, growth of S. mutans in saliva ranged <300 CFU/mL (0.1 nm) at concentrations of 25%, 12.5% and 6.25% for 24 hours. Whereas at the 48 h and 72 h period of incubation shown an increase in growth of S. mutans ranged 300-600 CFU/mL (0.2-0.36 nm). The inhibitory biofilm formation of S. mutans in saliva was significantly higher at concentrations of 12.5% and 6.25% at 24 h incubation times on a moderate scale, whereas the histatin-5 was effective to inhibit S. mutans biofilm on the 50 and 25 ppm. The saliva possessed a higher inhibitory of biofilm S. mutans than histatin-5 and good level viscosity (0.91-0.92 cP). Conclusion: The saliva was able to control the growth of S. mutans, and histatin-5 can inhibit the biofilm formation S. mutans. Furthermore, the saliva was also able to respond to the pH change with good viscosity of saliva.
BACKGROUND: Rheumatic fever and rheumatic heart disease (RHD) are the most common acquired heart diseases in children. Environmental factors have been reported to play an important role in RHD's prevalence.
AIM:The main purpose of this study is to assess the associations between environmental factors and RHD in children.
METHODS:A case-control study was conducted in the Department of Child Health, Haji Adam Malik Hospital from April to June 2017. The case group consisted of children aged 5-18 years with RHD while control group consisted of healthy children. Demographic, anthropometric, and laboratory data were collected along with environmental factors. Statistical analysis was done using Statistical Product and Service Solution. A P value of <0,05 with 95% confidence interval was considered significant.
RESULTS:A total of 39 children were enrolled in each group. Subjects' father who only went to elementary and junior high school had a higher risk of having children with RHD (OR 28; p = 0.032 and OR 15.75; p = 0.011, respectively). Subjects' mother who only went to junior high school had 7 times higher risk of having children with RHD (p = 0.026). Low monthly income increased the risk of RHD (OR 3,68; p = 0,009). Tap water usage, meat consumption more than once per week, and feasibility to buy clothes >1 pair per year decreased the risk of RHD at 0,31 (p = 0,013), 0,3 (p = 0,016), and 0,04 times (p <0,001), respectively. CONCLUSION: Parent's education, monthly family income, water source, frequency of meat consumption, and feasibility to buy clothes are related to RHD in children.
Psoriasis is a chronic inflammatory disease mediated by the immune system with a prevalence of 2-3% in the world population whose manifestations are found in the skin and other organs. Data on the prevalence of psoriasis is still diverse in several hospitals in Indonesia. Multifactors play a role in the incidence of psoriasis both genetic and environmental. Psoriasis lesions occur due to an imbalance between proliferation and differentiation of keratinocytes. Vitamin D has a role in regulating the balance of the process. Vitamin D is a fat soluble vitamin that can suppress the inflammatory process in psoriasis lesions.This study aimed to determine the frequency distribution of age, sex and levels of 25-Hydroxyvitamin D in blood plasma 44 psoriasis patients.Kadar vitamin D tersebut diukur menggunakan metode ELISA (DBC). The results obtained were the average age of 42-year-old psoriasis patients with the youngest age of 18 years and the oldest at 77 years. Female sex was 56.8% and men were 43.2%.The highest vitamin D level was 59.1 ng / ml and the lowest was 7.3 ng / ml so that it averaged 25.92 ng /ml. Analysis of vitamin D levels produced suficiency (31.8%), insufficiency (31.8%) and deficiency (36.4%).It can be concluded that vitamin D levels in psoriasis patients in Medan city 68.2% have less categories (<29 ng / ml) and most deficiencies at 48-57 years old.
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