The isolation of Stropharia rugosoannulata polysaccharide (SRP) by three-phase extraction was optimized, and its structure and biological activities were identified. The optimal extraction conditions were: mass fraction of ammonium sulfate, 20%; volume ratio of sample solution to t-butanol, 1:1.5; extraction temperature, 35°C. Under these conditions, the yield of SRP was 6.85% ± 0.13%. SRP was found to be composed of glucose (35.79%), galactose (26.80%), glucuronic acid (9.92%), fructose (8.65%), xylose (7.92%), fucose (4.19%), arabinose (3.46%) and rhamnose (3.26%), with the molecular weight of 27.52 kDa. The results of DPPH, hydroxyl, ABTS+ radical scavenging and reducing power tests showed that SRP had good antioxidant capacities. SRP had no cytotoxic effect on RAW264.7 macrophages at the concentrations of 25-200 μg/mL, and could significantly promote phagocytosis activity and cell migration according to CCK-8 assay, phagocytosis assay and cell scratch experiment. SRP can significantly stimulate the transcript expression levels of TNF-α, IL-1β and IL-6, as determined by RT-PCR and Western blot assays. SRP activated the TLR4/NF-κB signaling pathway, and autophagy also occurred. These results suggest that SRP is a safe antioxidant and immunomodulator, and that it can be used in the development of functional foods and/or pharmaceuticals.
Due to the effects of splitting frequency and cross coupling, the resonant frequency of the WPT system usually deviates from the given frequency band, and the system operating at the given frequency band suffers a very low output power. Ensuring that electric vehicle wireless power transfer (EV-WPT) systems operate at a resonant state is the prerequisite for efficient energy transfer. For this purpose, a novel design method by manipulating the eigenstate parameters is proposed in this paper. The proposed system can make a EV-WPT system with arbitrary coil successfully to resonate at any given bands, not just a single band. Therefore, the method designed in this article cannot only eliminate the problem of low power caused by frequency deviation, but also realize the application requirements of multiple frequency bands. Firstly, this article establishes an accurate state space model of an n-coil fully coupled EV-WPT system, and after that, the analytical current response on each circuit is derived. Based on that, a detailed frequency spectrum analysis is presented, along with several essential spectrum parameters’ derivations, including center frequencies and bandwidths. Then, with the center frequency and bandwidth as the design indexes, a novel methodology of designing to make EV-WPT systems achieve resonant-state at arbitrary given bands is derived. Finally, simulation and experimental verification are carried out. Simulation and experimental results show that whether it is a single-band or multi-band system, the accuracy of the value under designed resonant frequency is less than 0.01, which can effectively eliminate the frequency deviation phenomenon and obtain the maximum power output at the given frequency band.
In the rotary steering system of oil drilling, both power and data transmission are needed. This paper presents a parallel power and data transmission method for a rotating steering system with output voltage control. To reduce the size of the system, power and data are transmitted through the same rotational coupling mechanism. A power transfer resonance circuit is used to suppress the influence of the power transfer on data transmission. Therefore, crosstalk interference between the power and the data transmission channel is negligible. The experimental prototype is built, and the feasibility of the data transfer method and the closed-loop control method is verified. The experimental results are in good agreement with the theoretical analysis.
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