The purposes of this study were to investigate the effect of administration of GnRH and PGF2α on onset of estrus and conception rate in different postpartum swamp buffalo (Bubalus bubalis). Twenty postpartum (pp) buffalo-cows were divided into four groups of five buffaloes as follows: Group A (30 day pp), Group B (45 dpp), Group C (60 dpp), and Group D (75 dpp). The buffaloes in each group were injected intramuscularly with 3 ml GnRH at day 0, followed by intramuscular injection of 2.5 ml PGF2α, 10 ml) at day 7 after GnRH injection. Observation of estrus was performed 24 h after injection of PGF 2α. Artificial insemination was performed 18 h after estrus signs clearly visible. The result of study showed that there were no difference (p>0.05) among treatments on the percentages of estrus (100%), onset of estrus (37.4 to 38.4 h), duration of estrus (16.8 to 18.2 h) and conception rate (100%). In conclusion, buffalo-cows in Kampar Regency can be served as early as 30 days post partum using combination of 3 ml GnRH and 2.5 ml of PGF2α.
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