A patient with a squamous cell carcinoma accompanied by a marked granulocytosis ( 100,000/mm3) of unknown origin was examined for Colony-Stimulating Activity ( N PATIENTS WITH nonhematological malig-
Detection ofgranulocyte colony-stimulating factor (G-CSF), one of the substances responsible for proliferation and diEferentiation of granulocytes, has been performed up to the present by use of the granulocyte colony-formation assay, because of the lack ofa specific anti-G-CSF antibody. This has prevented the advancement of biological investigations of cell dynamics linked to G-CSF, e.g., cell localization of G-CSF and its pathophysiobogical changes. In the present work, two monoclonal antibodies (MAb), 1E7 and 4A6, against recombinant human G-CSF (rhG-CSF) were devel-o_ by cell hybridization between NS-1 myeboma cells and splenocytes from a mouse immunized with rhG-CSF. 1E7 and 4A6 were shown to be reactive with hG-CSF but not with other CSF (hGM-CSF, hIL-3, and mouse GM-CSF) by Western blot analysis.
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